TRAILR2/TNFRSF10B Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56618

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRAIL R2/TNFRSF10B Primary Antibodies »

Product Specifications

Immunogen

Rabbit anti-DR5 polyclonal antibody was raised against a peptide corresponding to amino acids 388 to 407 (isoform 2) of human DR5 precursor (1,2). The same sequence is found in isoform 1 at amino acids 417-436.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21474767).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAILR2/TNFRSF10B Antibody - BSA Free

Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618]

Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618]

Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Total protein from HL-60, HepG2, HCT-116 and human placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TRAIL R2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: TRAILR2/TNFRSF10B Antibody [NB100-56618]

Immunohistochemistry-Paraffin: TRAILR2/TNFRSF10B Antibody [NB100-56618]

Immunohistochemistry-Paraffin: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis of a formalin fixed paraffin-embedded (FFPE) human brain cerebellum using 1:200 conc. of TRAIL R2/TNFRSF10B antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Cytoplasmic staining was observed in the Purkinje cell layer.
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody [NB100-56618]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody [NB100-56618]

TRAILR2-TNFRSF10B-Antibody-Immunohistochemistry-NB100-56618-img0008.jpg
Immunocytochemistry/ Immunofluorescence: TRAILR2/TNFRSF10B Antibody [NB100-56618]

Immunocytochemistry/ Immunofluorescence: TRAILR2/TNFRSF10B Antibody [NB100-56618]

Immunocytochemistry/Immunofluorescence: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TRAIL R2 (H76) at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618]

Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618]

Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis using the Azide Free version of NB100-56618. Detection of 20 ug of whole cell lysates from HL60 cells with anti-TRAIL-R2 at 5 ug/ml.

Applications for TRAILR2/TNFRSF10B Antibody - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (KOYAMA (2003); PMID 16426839)

Flow Cytometry

1:20-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (Georgakis et al (2005))

Immunohistochemistry

1:200. Use reported in scientific literature (PMID 27740879)

Immunohistochemistry-Paraffin

reported in scientific literature (Younes et al (2006))

Western Blot

5 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB100-56618 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAIL R2/TNFRSF10B

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-2). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human)

Gene Symbol

TNFRSF10B

Additional TRAIL R2/TNFRSF10B Products

Product Documents for TRAILR2/TNFRSF10B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRAILR2/TNFRSF10B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRAILR2/TNFRSF10B Antibody - BSA Free

Customer Reviews for TRAILR2/TNFRSF10B Antibody - BSA Free (1)

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  • TRAIL R2/TNFRSF10B Antibody
    Name: Qing Xu
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 07/17/2018
    Total cell lysates from T47D and MDA-MB-231 cells were subjected to western blot. PVDF membrane were probed with 2.5 mm/ml Human TRAIL R2 (NB100-56618). A specific band was detected for TRAIL R2 at approximately 48~50 kDa. This experiment was conducted under reducing conditions
    TRAILR2/TNFRSF10B Antibody - BSA Free NB100-56618

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