Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85‑95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidnay tubules. Human Uromodulin is GPI‑linked. Its proprecursor is 616 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑149), a ZP domain that mediates oligomerization (aa 334‑589) and a cleavable C‑terminal propeptide (aa 615‑640). There are multiple splice variants. One shows a deletion of aa 67‑199, a second shows a nine aa substitution for aa 609‑640, a third shows a Pro substitution for aa 205‑234 and a fourth shows a 66 aa substitution for aa 613‑640. Over aa 25‑614, human Uromodulin is 78% aa identical to mouse Uromodulin.
Key Product Details
Species Reactivity
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Applications
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Label
Antibody Source
Product Specifications
Immunogen
Asp25-Ser614
Accession # P07911
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Uromodulin Antibody
Detection of Human Uromodulin by Western Blot.
Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Uromodulin at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Uromodulin by Simple WesternTM.
Simple Western shows lysates of Exosome Standards (Human Urine) (NBP2-49840) and human kidney tissue, loaded at 0.5 mg/ml. A specific band was detected for Uromodulin at approximately 149 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Uromodulin in Human Kidney.
Uromodulin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Applications for Human Uromodulin Antibody
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human kidney
Simple Western
Sample: Exosome Standards (Human Urine) (Catalog # NBP2-49840) and human kidney tissue
Western Blot
Sample: Human kidney tissue
Reviewed Applications
Read 1 review rated 5 using AF5144 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Uromodulin
Alternate Names
Gene Symbol
UniProt
Additional Uromodulin Products
Product Documents for Human Uromodulin Antibody
Product Specific Notices for Human Uromodulin Antibody
For research use only
Citations for Human Uromodulin Antibody
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Application: Western BlotSample Tested: UrineSpecies: RatVerified Customer | Posted 07/06/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars