VCAM-1/CD106 Antibody (1.G11B1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-28404

Clone 1.G11B1 was used by HLDA to establish CD designation.
Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Porcine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1.G11B1

Format

Azide and BSA Free
View all VCAM-1/CD106 Primary Antibodies »

Product Specifications

Immunogen

The immunogen is a peptide made to the amino acid region VCAM1

Reactivity Notes

Porcine reactivity reported from a verified customer review.

Specificity

Human CD106/VCAM-1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for VCAM-1/CD106 Antibody (1.G11B1) - Azide and BSA Free

Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404]

Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404]

VCAM-1-CD106-Antibody-1-G11B1-Western-Blot-NBP1-28404-img0007.jpg
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Veins and venules in the interalveolar stroma of porcine mammary gland are positive for the human VCAM-1. Antigen retrieval by Proteinase K. AlexaFluor 488 anti-mouse antibody was used as a secondary detection antibody. Image from verified customer review.
Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404]

Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404]

Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - TNF alpha stimulated human endothelial cell line HUV-EC-C was stained with Mouse Anti-Human CD106-UNLB followed by Goat Anti-Mouse IgG1, Human ads-FITC.
Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404]

Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404]

Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Analysis using the FITC conjugate of NBP1-28404. Staining of TNF alpha stimulated endothelial cell line HUV-EC-C.
VCAM-1/CD106 Antibody (1.G11B1)

Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] -

Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Knockdown of RPN2 decreased luminal B BC cell dissemination in vivo. Luminal B ER+ T47D cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting RPN2 (siRNA-RPN2) were injected + estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos with green fluorescent blood vessels & analyzed as described in materials & methods. (A) Migration in vitro (n = 6). (B)In vivo dissemination of transfected T47D in presence of E2 ± Neu (n = 23–26). Scale bar = 100 µm. (n = 23–26). (C) Western blot analysis for confirmation of siRNA-RPN2 transfection & VCAM-1, ICAM-1, & MUC-1 expression. (D) Focal adhesions area (n = 5–6). Scale bar = 10 µm. (E) Proliferation (n = 6). Representative images of zebrafish embryos with disseminated luminal B T47D BC cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated T47D & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, ***P < 0.001, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
VCAM-1/CD106 Antibody (1.G11B1)

Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] -

Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Knockdown of U2AF1 decreased luminal A BC cell dissemination in vivo. Luminal A ER+ MCF-7 cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting U2AF1 (siRNA-U2AF1) were injected in presence of estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos, with green fluorescent blood vessels, & analyzed as described in materials & methods. (A) Migration in vitro (n = 6–12). (B)In vivo dissemination in presence of E2 ± Neu (n = 38–41). Scale bar = 100 µm. (C) Western blot analysis for confirmation of siRNA-U2AF1 transfection & ICAM-1, VCAM-1, & MUC-1 expression. (D) Focal adhesion area (n = 7). Scale bar = 10 µm. (E) Proliferation in vitro (n = 12). Representative images of zebrafish embryos with disseminated MCF-7 cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated MCF-7 cells & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, **P < 0.01, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for VCAM-1/CD106 Antibody (1.G11B1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/10^6 cells

Immunohistochemistry-Paraffin

Validated from a verified customer review

Western Blot

1:100 - 1:2000

Reviewed Applications

Read 1 review rated 3 using NBP1-28404 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Borate buffered saline, pH 8.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VCAM-1/CD106

CD106, also known as INCAM-1, is a 100-110 kDa vascular adhesion cell adhesion molecule-1 (VCAM-1) that is member of the immunoglobulin superfamily. CD106 is expressed predominantly on cytokine activated vascular endothelium but has also been identified on interfollicular dendritic cells, some macrophages and bone marrow stromal cells. Endothelial CD106 binds the integrins alpha4beta1 (CD49d/CD29, VLA-4) and alpha4beta7 and contributes to extravasation of lymphocytes, monocytes, basophils and eosinophils (but not neutrophils) from blood vessels, particularly at sites of inflammation. Unlike the beta2 integrins, the CD106-VLA-4 interaction can mediate both the initial tethering and rolling of lymphocytes on endothelium as well as their subsequent arrest and firm adhesion. CD106 expressed on non-vascular tissues has been implicated in the interaction of hematopoietic progenitors with bone marrow stromal cells, B cell binding to follicular dendritic cells, costimulation of T cells, and embryonic development. The monoclonal antibody 1.G11B1 inhibits in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium. (1-4)

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (1.G11B1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VCAM-1/CD106 Antibody (1.G11B1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VCAM-1/CD106 Antibody (1.G11B1) - Azide and BSA Free

Customer Reviews for VCAM-1/CD106 Antibody (1.G11B1) - Azide and BSA Free (1)

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Customer Images

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  • VCAM-1/CD106 Antibody (1.G11B1)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mammary gland tissue
    Species: Porcine
    Verified Customer | Posted 06/28/2019
    Veins and venules in the interalveolar stroma of porcine mammary gland are positive for the human VCAM-1.
    Antigen retrieval by proteinase K. Alexa Fluor 488 anti-mouse antibody was used as a secondary detection antibody.
    VCAM-1/CD106 Antibody (1.G11B1) - Azide and BSA Free NBP1-28404

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