VCAM-1/CD106 Antibody (6G9) - BSA Free

Novus Biologicals | Catalog # NBP1-47491

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, ELISA, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6G9

Format

BSA Free
View all VCAM-1/CD106 Primary Antibodies »

Product Specifications

Immunogen

This VCAM-1/CD106 Antibody (6G9) was developed against a purified recombinant fragment of human VCAM-1 expressed in E. coli. [Uniprot: P19320]

Reactivity Notes

Use in Mouse reported in scientific literature (PMID: 32243809).

Localization

Membrane. Single-pass type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VCAM-1/CD106 Antibody (6G9) - BSA Free

Western Blot: VCAM-1/CD106 Antibody (6G9)BSA Free [NBP1-47491]

Western Blot: VCAM-1/CD106 Antibody (6G9)BSA Free [NBP1-47491]

Western Blot: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate.
Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-NBP1-47491-img0010.jpg
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - (A) human lung cancer and (B) colon cancer using VCAM1 mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Human placenta tissues using VCAM1 mouse mAb.
Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Breast carcinoma, cytoplasmic staining. IHC image submitted by a verified customer review.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - FFPE section of human lung cancer. Image at 20X magnification. IHC-P image submitted by a verified customer review.
Simple Western: VCAM-1/CD106 Antibody (6G9)BSA Free [NBP1-47491]

Simple Western: VCAM-1/CD106 Antibody (6G9)BSA Free [NBP1-47491]

Simple Western: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Lane view shows a specific band for CD106/VCAM1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
VCAM-1/CD106 Antibody (6G9) - BSA Free

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] -

Photomicrographs of two separate gut sections from a patient with EHEC colitis.Panels (A) and (B) are stained with CD31 to enumerate endothelium lining the vessels (40× magnification). (C) and (D) are stained to show VCAM-1 expression in endothelium, indicating inflammatory activation (40× magnification).
VCAM-1/CD106 Antibody (6G9) - BSA Free

Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] -

Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] - Enhanced expression of endothelial SENP1 & GATA2 correlates w/ graft arteriosclerosis (GA) progression.Similarly sized human coronary arteries w/ GA from chronically rejecting heart allografts or w/out disease from non-transplanted hearts collected & evaluated by histological analysis. (a,b) Dramatically increased expression of endothelial SENP1 detected in the diseased vessel wall. Endothelial SENP1 expression is demonstrated by immunofluorescence analysis of coronary artery cross-sections that stained for SENP1 & the endothelial marker PECAM-1 w/ DAPI labelling of the nuclei. Representative images are shown in (a) w/ quantification data in (b). Bar represents 50 μm. (c) Induction of endothelial adhesion molecules resulted in a similar augmented pattern as endothelial SENP1. Representative images of immunofluorescence staining for VCAM-1 or P-selectin & PECAM-1 in coronary arteries w/ DAPI counterstaining are shown. Bar represents 50 μm. (d,e) Expression of endothelial GATA2 elevated w/ the progression of aggravated rejection. Representative images of the immunofluorescence staining of coronary arteries for GATA2 & PECAM-1 together w/ DAPI nuclear staining are shown in (d) w/ quantification data in (e). Negative SENP1 & GATA2 staining in the isotype or blocking peptide controls are also shown in (a) & (d). Bar represents 50 μm. Data presented in (b,e) are the mean±s.e.m. from five separate clinical specimens per group as indicated. *P<0.05, **P<0.01 & ***P<0.0001; one-way ANOVA followed by Bonferroni test. GA, graft arteriosclerosis; PECAM, platelet/endothelial cell adhesion molecule; SENP, sentrin-specific protease; VCAM, vascular cell adhesion molecule. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms15426), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
VCAM-1/CD106 Antibody (6G9) - BSA Free

Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] -

Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] - Loss of endothelial SENP1 inhibits EC activation.(a) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin & PECAM-1 with DAPI labelling of the nuclei. Bar represents 50 μm. (b–e) Attenuated induction of adhesion molecules in SENP1-ecKO MAECs. Flow cytometry analysis of ICAM-1, VCAM-1 & P-selectin in MAECs isolated from WT or SENP1-ecKO mice after TNF or IL-1 beta treatment. Representative histograms are shown in (b) with the quantification of mean intensity in (c–e). (f–h) Overexpression of the catalytically inactive form of SENP1 (SENP1-Mut) inhibits the induction of adhesion molecules in HUVECs. HUVECs were infected by Ad-SENP1-Mut or vector control (Ad-LacZ) for 24 h, treated with pro-inflammatory cytokines & analysed by flow cytometry in the same way as MAECs. Representative histograms of ICAM-1 & VCAM-1 are shown in (f) with the quantification of mean intensity in (g,h). Data are presented as the mean±s.e.m. from at least three independent experiments. *P<0.05 & **P<0.01; two-way ANOVA followed by Bonferroni post-test. MAEC, mouse aortic endothelial cell. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms15426), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for VCAM-1/CD106 Antibody (6G9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunoblotting

reported in scientific literature (PMID 28569748)

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

reported in scientific literature (PMID 28569748)

Simple Western

1:1000

Western Blot

1:500-1:2000
Application Notes

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HUVEC lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 56 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-47491 in the following applications:

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VCAM-1/CD106

VCAM1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. Tissue specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human)

Gene Symbol

VCAM1

UniProt

P19320

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (6G9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VCAM-1/CD106 Antibody (6G9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VCAM-1/CD106 Antibody (6G9) - BSA Free

Customer Reviews for VCAM-1/CD106 Antibody (6G9) - BSA Free (2)

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  • VCAM-1/CD106 Antibody (6G9)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: FFPE and human lung cancer
    Species: Human
    Verified Customer | Posted 10/13/2020
    20X
    VCAM-1/CD106 Antibody (6G9) - BSA Free NBP1-47491
  • Name: Bryan Tinsley
    Application: Immunohistochemistry
    Sample Tested:
    Species: Human
    Verified Customer | Posted 04/21/2014
    Breast carcinoma: Cytoplasmic staining
    VCAM-1/CD106 Antibody (6G9) - BSA Free NBP1-47491

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