VEGF Antibody (VG76e) - BSA Free

Novus Biologicals | Catalog # NB100-648

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Rat, Porcine, Bovine, Goat, Sheep

Cited:

Human, Rat, Porcine, Bovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Radioimmunoassay

Cited:

Immunohistochemistry-Paraffin, Western Blot, Radioimmunoassay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # VG76e

Format

BSA Free
View all VEGF Primary Antibodies »

Product Specifications

Immunogen

Recombinant human VEGF189 expressed in E.coli [UniProt# P15692]

Reactivity Notes

Mixed results in Cow and Pig (IHC only). Customers have reported success staining sheep tissue (see review). Goat reactivity reported from a verified customer review.

Specificity

Using recombinant proteins, it was demonstrated that VG76e recognizes the 121, 165, and 189 isoforms of VEGF

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VEGF Antibody (VG76e) - BSA Free

Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) - BSA Free [NB100-648]

Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) - BSA Free [NB100-648]

Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) [NB100-648] - IHC analysis of VEGF in fetal sheep brain tissue. Image courtesy of anonymous customer review.
Western Blot: VEGF Antibody (VG76e)BSA Free [NB100-648]

Western Blot: VEGF Antibody (VG76e)BSA Free [NB100-648]

Western Blot: VEGF Antibody (VG76e) [NB100-648] - Analysis of VEGF in human kidney protein (30ug).
Immunocytochemistry/ Immunofluorescence: VEGF Antibody (VG76e) - BSA Free [NB100-648]

Immunocytochemistry/ Immunofluorescence: VEGF Antibody (VG76e) - BSA Free [NB100-648]

Immunocytochemistry/Immunofluorescence: VEGF Antibody (VG76e) [NB100-648] - Immunofluorescent analysis of VEGF using NB100-648. This image submitted through a verified customer review.
Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) - BSA Free [NB100-648]

Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) - BSA Free [NB100-648]

Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) [NB100-648] - IHC-P analysis of VEGF in sheep tonsil using NB100-648. This image was submitted through a verified customer review.
VEGF Antibody (VG76e) - BSA Free

Western Blot: Mouse Monoclonal VEGF Antibody (VG76e) [NB100-648]

Western Blot: Mouse Monoclonal VEGF Antibody (VG76e) [NB100-648] - Bone lysate homogenate from goats. SDS-PAGE with 0.3 mg of total protein from the homogenate per well. VEGF, NB100-648, was used at a dilution of 1:1000 in TBS Tween and 5% milk powder. The protein was labeled at 40 kDa. Quantification of VEGFA in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. Evaluation of pre-cellularized graft in iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to tibial bone defect, G2: cellularized graft for 6 weeks applied to tibial bone defect, G3: cellularized graft for 8 weeks applied to tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 120 days after graft implantation. Image from a verified customer review.
VEGF Antibody (VG76e) - BSA Free

Immunohistochemistry-Paraffin: Mouse Monoclonal VEGF Antibody (VG76e) [NB100-648]

The sections of histological sections of goat fragments were deparaffinized, peroxidase blocked in methanol and 30% hydrogen peroxide (9:1), and antigen retrieval was performed with porcine gastric pepsin and HCl at pH 3.0 in an oven at 37°C for 30 minutes. Then, blocking with BSA was performed for 1 hour followed by incubation with primary antibody VEGF antibody NB100-648 (1:100) overnight. Subsequently, excess primary antibody was removed with PBS pH 7.6 and incubated with secondary antibody (goat anti-mouse IgG - NB7539 (1:200) for 1 hour and application of polymer for 30 minutes. Finally, 150 μL of DAB was applied for five minutes and counterstained with hematoxylin. Image from a verified customer review.

Applications for VEGF Antibody (VG76e) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Radioimmunoassay

reported in scientific literature

Western Blot

0.5-2ug/ml
Application Notes
In Western blot a band is observed between 38-44 kDa. With IHC-paraffin sections, microwave treatment with Tris HCl pH 10 buffer (NOT citrate buffer) is necessary. VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each.

Reviewed Applications

Read 7 reviews rated 4.1 using NB100-648 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGF

Vascular endothelial growth factor (VEGF), also called VEGF-A and vascular permeability factor (VPF), is a secreted homodimeric glycoprotein belonging to the VEGF family with a role in stimulating angiogenesis and vasculogenesis (1,2). More specifically, VEGF-A secretion from most cell types contributes to promoting endothelial cell proliferation and migration, inhibiting apoptosis, increasing vascular permeability, and wound healing (1). The VEGF family consists of several members including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, VEGF-F, and placenta growth factor (PLGF) (1-4). As a result of alternative splicing of the eight exon VEGFA gene, there are several VEGF-A protein isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length, with VEGF121 and VEGF165 being the two most expressed isoforms (1,5). Full length VEGF-A monomer has a 26 aa signal sequence plus a 206 aa (VEGF206) sequence, with a theoretic molecular weight (MW) of 27 kDa, containing VEGF receptor 1 (VEGFR1) and VEGR2 binding sites and heparin-binding domains (1-3,5,6). VEGF121 lacks heparin affinity and binds the receptor tyrosine kinases (RTKs) VEGFR1 and VEGFR2, whereas VEGF165 has moderate affinity for heparin and, in addition to being a ligand for VEGFR1 and VEGFR2, can also bind the co-receptors neuropilin 1 (NRP1) and NRP2 (1,5). Hypoxia and hypoxia-related genes such as HIF-1, EGF, and PDGF are major regulators angiogenesis and VEGF expression (1,3). VEGF signaling initiated by ligand binding to its receptors results in activation of different pathways including PI3K and MAPK and ultimately guides endothelial cell proliferation, migration, and survival (1,3). While VEGF plays an important role in promoting normal angiogenesis and blood vessel formation, its expression is often upregulated in tumors and other angiogenesis-related pathologies like osteroarthritis (OA) (1-5,7). Given its function, VEGF and its receptors have become a therapeutic target for treating cancer and blocking angiogenesis (4,5,7). A recombinant humanized monoclonal anti-VEGFA antibody called bevacizumab (Avastin) was first approved by the FDA in 2004 for the treatment of a number of cancers (1-3,5). Cancer patients may experience resistance to anti-VEGF antibodies and, as such, clinical studies are exploring combination treatment options with chemotherapies and immune-checkpoint inhibitors (3,5).

References

1. Melincovici CS, Bosca AB, susman S, et al. Vascular endothelial growth factor (VEGF) - key factor in normal and pathological angiogenesis. Rom J Morphol Embryol. 2018;59(2):455-467.

2. Shaik F, Cuthbert GA, Homer-Vanniasinkam S, Muench SP, Ponnambalam S, Harrison MA. Structural Basis for Vascular Endothelial Growth Factor Receptor Activation and Implications for Disease Therapy. Biomolecules. 2020;10(12):1673. https://doi.org/10.3390/biom10121673

3. Apte RS, Chen DS, Ferrara N. VEGF in Signaling and Disease: Beyond Discovery and Development. Cell. 2019;176(6):1248-1264. https://doi.org/10.1016/j.cell.2019.01.021

4. Matsumoto K, Ema M. Roles of VEGF-A signalling in development, regeneration, and tumours. J Biochem. 2014;156(1):1-10. https://doi.org/10.1093/jb/mvu031

5. Itatani Y, Kawada K, Yamamoto T, Sakai Y. Resistance to Anti-Angiogenic Therapy in Cancer-Alterations to Anti-VEGF Pathway. Int J Mol Sci. 2018;19(4):1232. Published 2018 Apr 18. doi:10.3390/ijms19041232

6. Uniprot (P15692)

7. Hamilton JL, Nagao M, Levine BR, Chen D, Olsen BR, Im HJ. Targeting VEGF and Its Receptors for the Treatment of Osteoarthritis and Associated Pain. J Bone Miner Res. 2016;31(5):911-924. https://doi.org/10.1002/jbmr.2828

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human)

Gene Symbol

VEGFA

UniProt

P15692

Additional VEGF Products

Product Documents for VEGF Antibody (VG76e) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VEGF Antibody (VG76e) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VEGF Antibody (VG76e) - BSA Free

Customer Reviews for VEGF Antibody (VG76e) - BSA Free (7)

4.1 out of 5
7 Customer Ratings
5 Stars
43%
4 Stars
29%
3 Stars
29%
2 Stars
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1 Stars
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Customer Images

Showing  1 - 5 of 7 reviews Showing All
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  • VEGF Antibody (VG76e) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Bone and Bone Extracts
    Species: Goat
    Verified Customer | Posted 07/29/2025
    The f histological goat bone fragments were incubated with the primary antibody VEGF NB100-648 overnight. Subsequently, the secondary antibody goat anti-mouse IgG - NB7539 was applied and incubated for 1 hour.
    Validation of an immunohistochemistry technique for goats
    VEGF Antibody (VG76e) - BSA Free NB100-648
  • VEGF Antibody (VG76e) - BSA Free
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Bone and Bone Extracts
    Species: Goat
    Verified Customer | Posted 05/13/2025
    Bone lysate homogenate from goats. SDS-PAGE with 0.3 mg of total protein from the homogenate per well. VEGF, NB100-648, was used at a dilution of 1:1000 in TBS Tween and 5% milk powder. The protein was labeled at 40 kDa.
    Quantification of VEGFA in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. Evaluation of pre-cellularized graft in iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to tibial bone defect, G2: cellularized graft for 6 weeks applied to tibial bone defect, G3: cellularized graft for 8 weeks applied to tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 120 days after graft implantation.
    VEGF Antibody (VG76e) - BSA Free NB100-648
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Name: Hima S
    Application: Western Blot
    Sample Tested: Bovine Lysate
    Species: Other
    Verified Customer | Posted 09/14/2014
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: sheep tonsil
    Species: Other
    Verified Customer | Posted 07/29/2014
    Vegf in sheep tonsil
    VEGF Antibody (VG76e) - BSA Free NB100-648
  • Name: Ewa Kaniewska
    Application: Immunofluorescence
    Sample Tested:
    Species: Other
    Verified Customer | Posted 05/13/2014
    VEGF Antibody (VG76e) - BSA Free NB100-648
  • Name: Ewa Kaniewska
    Application: Immunocytochemistry
    Sample Tested: pig endothelial cells
    Species: Other
    Verified Customer | Posted 05/13/2014
  • Name: Ana Baburamani
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Fetal Sheep Brain Tissue
    Species: Other
    Verified Customer | Posted 05/04/2011
    VEGF Antibody (VG76e) - BSA Free NB100-648

There are no reviews that match your criteria.

Showing  1 - 5 of 7 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGF Antibody (VG76e) - BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Can you tell me if NB100-648 was raised against a peptide sequence of the mature VEGF protein, or against the whole protein? Do you know what aspect of the protein structure of VEGF that the antibody recognizes? Can you tell me if the antibody will detect VEGF-A isoforms such as 110,121,145,148, etc?

    A: The lab have informed me that the immunogen used was full length VEGF (subtype 189) protein. Unfortunately we have not tested if this detects all isoforms of VEGF-A.

  • Q: I have a question about your VEGF (AF214570) and its antibody (NB100-648). How long are their expiry date?

    A: We guarantee our antibodies for 6 months from the date of purchase, but they should function much longer than that.

  • Q: What is the heparin binding activity of FGF and VEGF?

    A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

  • Q: Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?

    A: I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

  • Q: Can you tell me if NB100-648 was raised against a peptide sequence of the mature VEGF protein, or against the whole protein? Do you know what aspect of the protein structure of VEGF that the antibody recognizes? Can you tell me if the antibody will detect VEGF-A isoforms such as 110,121,145,148, etc?

    A: The lab have informed me that the immunogen used was full length VEGF (subtype 189) protein. Unfortunately we have not tested if this detects all isoforms of VEGF-A.

  • Q: I have a question about your VEGF (AF214570) and its antibody (NB100-648). How long are their expiry date?

    A: We guarantee our antibodies for 6 months from the date of purchase, but they should function much longer than that.

  • Q: What is the heparin binding activity of FGF and VEGF?

    A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

  • Q: Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?

    A: I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

  • Q: Can you tell me if NB100-648 was raised against a peptide sequence of the mature VEGF protein, or against the whole protein? Do you know what aspect of the protein structure of VEGF that the antibody recognizes? Can you tell me if the antibody will detect VEGF-A isoforms such as 110,121,145,148, etc?

    A: The lab have informed me that the immunogen used was full length VEGF (subtype 189) protein. Unfortunately we have not tested if this detects all isoforms of VEGF-A.

  • Q: I have a question about your VEGF (AF214570) and its antibody (NB100-648). How long are their expiry date?

    A: We guarantee our antibodies for 6 months from the date of purchase, but they should function much longer than that.

  • Q: What is the heparin binding activity of FGF and VEGF?

    A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

  • Q: Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?

    A: I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

  • Q: Can you tell me if NB100-648 was raised against a peptide sequence of the mature VEGF protein, or against the whole protein? Do you know what aspect of the protein structure of VEGF that the antibody recognizes? Can you tell me if the antibody will detect VEGF-A isoforms such as 110,121,145,148, etc?

    A: The lab have informed me that the immunogen used was full length VEGF (subtype 189) protein. Unfortunately we have not tested if this detects all isoforms of VEGF-A.

  • Q: I have a question about your VEGF (AF214570) and its antibody (NB100-648). How long are their expiry date?

    A: We guarantee our antibodies for 6 months from the date of purchase, but they should function much longer than that.

  • Q: What is the heparin binding activity of FGF and VEGF?

    A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

  • Q: Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?

    A: I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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