VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free

Name: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP1-18646
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-18646

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 20I6

Format

Azide and BSA Free

View all VEGFR2/KDR/Flk-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human soluble extracellular KDR (D7)

Marker

Endothelial Cell Marker

Specificity

The monoclonal antibody will detect native human VEGFR-2/KDR in ELISA experiments and on the surface of different human cell types.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Clone #4 changed to 20I6 for a more unique identifier.

Scientific Data Images for VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free

Western Blot: VEGFR2/KDR/Flk-1 Antibody (20I6)Azide and BSA Free [NBP1-18646]

Western Blot: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - VEGFR2/KDR/Flk-1 Antibody (20I6) [NBP1-18646] - Recombinant human endogenous soluble VEGFR-2/KDR (esKDR) was produced in insect cells. Western blot was performed using our monoclonal anti-VEGFR-2 recognizing the soluble as well as the transmembrane form of KDR (left panal) and our new poyclonal antibody directed against the unique C-terminal end of the endogenous sKDR (CGRETILDHSAEAVGMP) recognizing solely the endogenous form but not sKDR (D7) and sKDR (D7)-Fc consisting of the full extraplasmatic domain. The endogenous sKDR generated by alternative splicing consists of the first 6 Ig-like loops with a unique c-terminal end.

Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]

Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - HMEC-1 CRL-3243 cells, stained with 1:50 VEGFR2/KDR/Flk-1 antibody (NBP1-18645AF488) (green) and DAPI (blue). Image from verified customer review.

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - VEGFR2/KDR/Flk-1 Antibody (20I6) [NBP1-18646] - Analysis with primary human dermal lymphatic endothelial cells(HDLEC).

Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]

Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - VEGFR2/KDR/Flk-1 Antibody (20I6) [NBP1-18646] - Staining (green) of VEGFR-2 (KDR) in primary human umbilical vein endothelial cells (HUVEC) with anti-human VEGFR-2 (Cl.4) (7,5ug/ml) and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:600.

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Applications for VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free

Application

Recommended Usage

ELISA

1-10 ug/ml

Flow Cytometry

2-5 ug/ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1:10-1:500

Western Blot

2-5 ug/ml

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VEGFR2/KDR/Flk-1

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Entrez Gene IDs

3791 (Human)

Gene Symbol

KDR

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.