Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat, Bovine, Canine, Chicken, Equine, Feline, Sheep

Cited:

Human, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # V9

Format

BSA Free
View all Vimentin Primary Antibodies »

Product Specifications

Immunogen

This antibody is a mouse monoclonal IgG1 antibody derived by fusion of PAI Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with vimentin isolated from porcine lens.

Reactivity Notes

Reacts with Potoroo. Canine reactivity reported in scientific literature (PMID: 24664167). Feline, Bovine, and Equine reactivity reported from a verified customer review.

Marker

Mesenchymal Cells Marker

Specificity

This antibody reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Vimentin Antibody (V9) - BSA Free

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Vimentin-Antibody-V9-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0006.jpg
Immunohistochemistry-Frozen: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Immunohistochemistry-Frozen: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Immunohistochemistry-Frozen: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of connective tissue cells in a frozen tissue section of human colon. No reactivity in epithelial cells.
Flow Cytometry: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Flow Cytometry: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - An intracellular stain was performed on Jurkat cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of glomeruli and connetive tissue in human kidney with in a 1:200 dilution. No reactivity in epithelial cells.
Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of connective tissue cells in swine colon with in a 1:200 dilution. No reactivity in epithelial cells.
Flow Cytometry: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Flow Cytometry: Vimentin Antibody (V9) - BSA Free [NBP1-97670]

Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - Analysis of PE conjugate of NBP1-97670. An intracellular stain was performed on THP-1 cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% s
Vimentin Antibody (V9) - BSA Free

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] -

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in human tonsil. NBP1-97670 was diluted to 0.5ug/mL and was left on sections for 30m at room temperature. Secondary antibody was Horse-Anti Mouse HRP Veterinary Reagent. Image from verified customer review.
Vimentin Antibody (V9) - BSA Free

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] -

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in horse intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on slides for 30m at room temperature. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent. Image from verified customer review.
Vimentin Antibody (V9) - BSA Free

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] -

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in feline intestine. NBP1-97670 was used at a concentration of 0.5ug/mL. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent. Image from verified customer review.
Vimentin Antibody (V9) - BSA Free

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] -

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in canine intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on tissue sections for 30m at room temperature. Secondary was Horse Anti-Mouse Veterinary Reagent. Image from verified customer review.
Vimentin Antibody (V9) - BSA Free

Immunohistochemistry-Paraffin: Mouse Monoclonal Vimentin Antibody (V9) [NBP1-97670] -

Immunohistochemistry-Paraffin: Mouse Monoclonal Vimentin Antibody (V9) [NBP1-97670] - Vimentin was stained in human FFPE tonsil tissue. HIER antigen retrieval at pH 9 for 20min was performed. Concentration used: 1:100. AF750 conjugated version of the antibody was used (Catalog # NBP1-97670AF750). Image from a verified customer review.

Vimentin Antibody (V9) - BSA Free

Immunohistochemistry-Paraffin: Mouse Monoclonal Vimentin Antibody (V9) [NBP1-97670]

Vimentin immunoreactivity in an FFPE section of cow lymph node. NBP1-97670 was diluted to 0.5ug/mL and was left on tissue sections for 30min at room temperature. Image from a verified customer review.

Applications for Vimentin Antibody (V9) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:200

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:25-1:200

Immunohistochemistry-Paraffin

1:25-1:200

Western Blot

1:100-1:1000
Application Notes
For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutes in 10 mM citrate buffer (pH 6.0) is required. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

This antibody is CyTOF ready..

Reviewed Applications

Read 5 reviews rated 5 using NBP1-97670 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH 7.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vimentin

Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

Alternate Names

VIM

Gene Symbol

VIM

Additional Vimentin Products

Product Documents for Vimentin Antibody (V9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Vimentin Antibody (V9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Vimentin Antibody (V9) - BSA Free

Customer Reviews for Vimentin Antibody (V9) - BSA Free (5)

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Showing  1 - 5 of 5 reviews Showing All
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  • Vimentin Antibody (V9) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Lymph Node
    Species: Cow
    Verified Customer | Posted 06/30/2025
    Vimentin immunoreactivity in an FFPE section of cow lymph node. NBP1-97670 was diluted to 0.5ug/mL and was left on tissue sections for 30min at room temperature.
    Vimentin Antibody (V9) - BSA Free NBP1-97670
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Vimentin Antibody (V9) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Tonsil
    Species: Human
    Verified Customer | Posted 06/05/2023
    Vimentin immunoreactivity in human tonsil. NBP1-97670 was diluted to 0.5ug/mL and was left on sections for 30m at room temperature. Secondary antibody was Horse-Anti Mouse HRP Veterinary Reagent.
    Vimentin Antibody (V9) - BSA Free NBP1-97670
  • Vimentin Antibody (V9) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Intestine
    Species: Equine
    Verified Customer | Posted 06/05/2023
    Vimentin immunoreactivity in horse intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on slides for 30m at room temperature. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent.
    Vimentin Antibody (V9) - BSA Free NBP1-97670
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Vimentin Antibody (V9) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Intestine
    Species: Feline
    Verified Customer | Posted 06/05/2023
    Vimentin immunoreactivity in feline intestine. NBP1-97670 was used at a concentration of 0.5ug/mL. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent.
    Requires heat-induced epitope retrieval. I used Target Retrieval Solution heated to 95 degrees Celsius using a vegetable steamer. Slides were left in pre-heated solution for 20m.
    Vimentin Antibody (V9) - BSA Free NBP1-97670
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Vimentin Antibody (V9) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Intestine
    Species: Canine
    Verified Customer | Posted 06/05/2023
    Vimentin immunoreactivity in canine intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on tissue sections for 30m at room temperature. Secondary was Horse Anti-Mouse Veterinary Reagent.
    Vimentin Antibody (V9) - BSA Free NBP1-97670

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