Detects viral MCV-type II Chemokine-like Protein in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with ________________ is observed.
Polyclonal Goat IgG
E. coli-derived recombinant viral MCV-type II Chemokine-like Protein
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Viral MCV-type II Chemokine-like Protein (Catalog # 697-M3)
Measured by its ability to neutralize MCV‑type II Chemokine‑like Protein inhibition of CCL1/I‑309-dependent chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 0.2 µg/mL Recombinant Viral MCV‑type II Chemokine‑like Protein and 20 #ng/mL Recombinant Human CCL1/I‑309.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
MCV‑type II Chemokine‑like Protein Inhibition of CCL1/I‑309-dependent Chemotaxis and Neutralization by Viral MCV‑type II Chemokine‑like Protein Antibody.
Recombinant Viral MCV‑type II Chemokine‑like Protein (Catalog # 697-M3) inhibits Recombinant Human CCL1/I‑309 (Catalog # 272-I) induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human CCL1/I‑309 (20 ng/mL) activity elicited by Recombinant Viral MCV‑type II Chemokine‑like Protein (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral MCV-type II Chemokine-like Protein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF697). The ND50 is typically 5-20 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCV-type II Chemokine-like Protein
Molluscum contagiosum virus (MCV) is a human cutaneous poxvirus that has been shown to encode homologs of cellular immunomodulatory proteins. MC148R2 is a CC chemokine-like protein encoded by an MCV type II gene. MC148R2 is a 104 amino acid (aa) protein containing a 24 aa signal peptide that is cleaved to produce an 80 aa mature protein.
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