Wnt-3a Antibody (HL1911) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25753

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1911 expressed in HEK293

Format

Azide and BSA Free
View all Wnt-3a Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Wnt-3a.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-3a Antibody (HL1911) - Azide and BSA Free

Wnt-3a Antibody (HL1911) - Azide and BSA Free

Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] -

Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Wnt-3a Antibody (HL1911) - Azide and BSA Free

Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] -

Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Wnt-3a Antibody (HL1911) - Azide and BSA Free

Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] -

Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Wnt-3a Antibody (HL1911) - Azide and BSA Free

ICC/IF Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] -

ICC/IF Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: Wnt3a stained by Wnt3a antibody [HL1911] diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10μm.
Wnt-3a Antibody (HL1911) - Azide and BSA Free

Immunohistochemistry-Paraffin Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] -

Immunohistochemistry-Paraffin Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Rat tissues.Wnt3a stained by Wnt3a antibody [HL1911] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wnt-3a Antibody (HL1911) - Azide and BSA Free

Immunohistochemistry-Paraffin Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] -

Immunohistochemistry-Paraffin Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Human prostate cancer.Wnt3a stained by Wnt3a antibody [HL1911] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for Wnt-3a Antibody (HL1911) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-3a

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Anti-human Wnt3a mAb, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.

Long Name

Wingless-type MMTV Integration Site Family, Member 3a

Alternate Names

Wnt3a

Gene Symbol

WNT3A

Additional Wnt-3a Products

Product Documents for Wnt-3a Antibody (HL1911) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Wnt-3a Antibody (HL1911) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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