Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 55010
View all Wnt-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-4
aa 37-76 and 222-295
Accession # P22724

Specificity

Detects mouse Wnt-4 in direct ELISAs and Western blots. In sandwich immunoassays, this antibody is specific for human Wnt-4 as a capture antibody when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Wnt-4 Antibody (55010) [DyLight 350]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Wnt-4

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and DAX1, a gene known to antagonize the testis-determining factor, play a concerted role in both the control of female development and the prevention of testes formation. WNT4 and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue.

Long Name

Wingless-type MMTV Integration Site Family, Member 4

Alternate Names

Wnt4

Gene Symbol

WNT4

Additional Wnt-4 Products

Product Documents for Wnt-4 Antibody (55010) [DyLight 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Wnt-4 Antibody (55010) [DyLight 350]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Ligands (Wnts)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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