Human/Mouse Wnt-4 Antibody

Catalog # Availability Size / Price Qty
Wnt‑4 in Mouse Embryo.
2 Images
Product Details
Citations (3)
Supplemental Products

Human/Mouse Wnt-4 Antibody Summary

Species Reactivity
Human, Mouse
Detects mouse Wnt-4 in direct ELISAs and Western blots. In sandwich immunoassays, this antibody is specific for human Wnt-4 as a capture antibody when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076)
Monoclonal Rat IgG2A Clone # 55010
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Wnt-4
aa 37-76 and 222-295
Accession # P22724
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse Wnt‑4 (Catalog # 475-WN)

This antibody functions as an ELISA capture antibody for Human Wnt-4 when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076).

This product is intended for assay development on various assay platforms requiring antibody pairs.

8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Wnt-4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Wnt‑4 in Mouse Embryo. Wnt-4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rat Anti-Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB475) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Human Wnt-4 ELISA Standard Curve. Recombinant Human Wnt-4 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB475) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-4

Wnt-4 is a secreted glycoprotein that binds to receptors of the Frizzled family. Wnt-4 is important for multiple embryonic development processes including kidney morphogenesis.

Long Name
Wingless-type MMTV Integration Site Family, Member 4
Entrez Gene IDs
54361 (Human); 22417 (Mouse)
Alternate Names
protein Wnt-4; SERKAL; wingless-type MMTV integration site family, member 4; Wnt4; Wnt-4

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Citations for Human/Mouse Wnt-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism
    Authors: LY Hung, D Sen, TK Oniskey, J Katzen, NA Cohen, AE Vaughan, W Nieves, A Urisman, MF Beers, MF Krummel, DR Herbert
    Mucosal Immunol, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
    Authors: Shi, Yuqiang, Shu, Jianhong, Gelman, Benjamin, Lisinicchia, Joshua G, Tang, Shao-Jun
    J Neuroimmune Pharmacol, 2013;8(4):956-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Wnt glycoproteins regulate the expression of FoxN1, the gene defective in nude mice.
    Authors: Balciunaite G, Keller MP, Balciunaite E, Piali L, Zuklys S, Mathieu YD, Gill J, Boyd R, Sussman DJ, Hollander GA
    Nat. Immunol., 2002;3(11):1102-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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