![Western Blot: Wnt-4 Antibody [NB100-1006]](https://resources.rndsystems.com/images/products/Wnt-4-Antibody-Western-Blot-NB100-1006-img0002.jpg "Western Blot: Wnt-4 Antibody [NB100-1006]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-SNWLYLAKLSSVGS corresponding to internal region according to NP_110388.2.
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Rat.
Localization
Possibly secreted and associates with the extracellular matrix.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Wnt-4 Antibody
Western Blot: Wnt-4 Antibody [NB100-1006]
Western Blot: Wnt-4 Antibody [NB100-1006] - (1ug/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: Wnt-4 Antibody [NB100-1006]](https://resources.rndsystems.com/images/products/Wnt-4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1006-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Wnt-4 Antibody [NB100-1006]")
Immunocytochemistry/ Immunofluorescence: Wnt-4 Antibody [NB100-1006]
Immunocytochemistry/Immunofluorescence: Wnt-4 Antibody [NB100-1006] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue)Applications for Wnt-4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
Detection limit 1:4000
Western Blot
1:100-1:2000
Application Notes
WB: Approx. 40+35 kDa band observed in Jurkat lysates (calculated MW of 39.1 kDa band according to NP_110388.2 Negative Control: U937 cell lysate.).
Immunofluorescence: Strong expression of the protein seen in the Vesicle of HeLa and MCF7 cells.
Immunofluorescence: Strong expression of the protein seen in the Vesicle of HeLa and MCF7 cells.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Wnt-4
Long Name
Wingless-type MMTV Integration Site Family, Member 4
Alternate Names
Wnt4
Gene Symbol
WNT4
UniProt
Additional Wnt-4 Products
Product Documents for Wnt-4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Wnt-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
