YAP1 Antibody (1A12) - BSA Free

A: YAP1 or Yes-associated protein YAP65 homolog has many phosphorylation sites which can affect the mass.  While the theoretical weight is 54.5 kDa, the “65” in YAP65 is the mass which has historically been associated with the protein. Before looking at your electrophoresis and gel conditions, I would focus on your cell growth conditions. Each experiment must start with the same number of cells to seed the media and the timing of harvesting and lysing your cells must be consistent between experiments.  Variation in the density of cells can affect YAP1 phosphorylation thus changing the mass of the protein.  In addition, there are 9 isoforms of YAP1 which can cause mass variations depending on cellular treatments, growth condition, cell lines and the specificity of the antibody you are using. I hope you find this useful in getting a consistent mass between experiments.