Key Product Details

Species Reactivity

Multi-Species

Applications

Immunohistochemistry, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Janelia Fluor 525

Antibody Source

Monoclonal Mouse IgG2A Clone # BU-1
View all Bromodeoxyuridine/BrdU Primary Antibodies »

Product Specifications

Immunogen

5-iodourindine (5-IO) coupled to ovalbumin

Specificity

Detects Bromodeoxyuridine/BrdU.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Bromodeoxyuridine/BrdU Antibody (BU-1) [Janelia Fluor® 525]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Bromodeoxyuridine/BrdU

Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.

Alternate Names

5-bromo-2-deoxyuridine, BrdU

Additional Bromodeoxyuridine/BrdU Products

Product Documents for Bromodeoxyuridine/BrdU Antibody (BU-1) [Janelia Fluor® 525]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (BU-1) [Janelia Fluor® 525]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Bromodeoxyuridine/BrdU Antibody (BU-1) [Janelia Fluor® 525]

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    • Q: I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
      A: We recommend this product: NB500-235
Showing  1 - 1 的 1 FAQ Showing All
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