CD44 Antibody (OX-50) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80649
![Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]](https://resources.rndsystems.com/images/products/CD44-Antibody-OX-50-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80649-img0001.jpg "Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OX-50
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Rat T cell blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells.
Localization
Type I membrane protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD44 Antibody (OX-50) - Azide and BSA Free
Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]
Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was detected using HRP-conjugated anti-Mouse secondary antibody with DAB reagent followed by hematoxylin counterstaini![Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]](https://resources.rndsystems.com/images/products/CD44-Antibody-OX-50-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80649-img0002.jpg "Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]")
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.![Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]](https://resources.rndsystems.com/images/products/CD44-Antibody-OX-50-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80649-img0003.jpg "Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]")
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.Applications for CD44 Antibody (OX-50) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:50 - 1:100
Immunohistochemistry
1:50 - 1:100
Immunohistochemistry-Frozen
1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
Immunohistochemistry-Paraffin
1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
Western Blot
reported in scientific literature (PMID 8537452)
Application Notes
This antibody is CyTOF ready.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Gene Symbol
CD44
Additional CD44 Products
Product Documents for CD44 Antibody (OX-50) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD44 Antibody (OX-50) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for CD44 Antibody (OX-50) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review CD44 Antibody (OX-50) - Azide and BSA Free and earn rewards!
Have you used CD44 Antibody (OX-50) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
