Fibronectin Antibody (2F4) - BSA Free

Novus Biologicals | Catalog # NBP2-22113

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Hydrolysis Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F4

Format

BSA Free
View all Fibronectin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Fibronectin expressed in E. coli. [UniProt# P02751]

Marker

Mesenchymal Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

262.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Fibronectin Antibody (2F4) - BSA Free (NBP2-22113) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-Fibronectin Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Fibronectin Antibody (2F4) - BSA Free

Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and permeabilized using PBS with 0.1% Triton X-100 before incubating them with Fibronectin antibody or the mouse IgG1 isotype control antibody. DyLight 488 conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody was used at 1: 200 dilution to detect the antigen-primary antibody complexes and the cells were counterstained with DAPI for labeling the nuclei. This Fibronectin antibody generated the expected punctate cytoplasmic-membranous staining and the signal was localized more to the inter-cellular spaces in clustered cells.
Western Blot: Fibronectin Antibody (2F4)BSA Free [NBP2-22113]

Western Blot: Fibronectin Antibody (2F4)BSA Free [NBP2-22113]

Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa (1) and HepG2 (2) whole cell lysates using Fibronectin antibody (2F4) at 2 ug/mL.
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded stomach cancer tissues using Fibronectin mouse mAb with DAB staining.
Flow Cytometry: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Flow Cytometry: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Flow Cytometry: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa cells using Fibronectin mouse mAb (green) and negative control (red).
Western Blot: Fibronectin Antibody (2F4)BSA Free [NBP2-22113]

Western Blot: Fibronectin Antibody (2F4)BSA Free [NBP2-22113]

Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against human Fibronectin (AA: 1965-2176) recombinant protein. Expected MW is 49.6 kDa.
Western Blot: Fibronectin Antibody (2F4)BSA Free [NBP2-22113]

Western Blot: Fibronectin Antibody (2F4)BSA Free [NBP2-22113]

Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against HEK293 (1) and Fibronectin (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded lung cancer tissues using Fibronectin mouse mAb with DAB staining.
ELISA: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

ELISA: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

ELISA: Fibronectin Antibody (2F4) [NBP2-22113] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng).

Applications for Fibronectin Antibody (2F4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200 - 11:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibronectin

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Entrez Gene IDs

2335 (Human)

Gene Symbol

FN1

UniProt

P02751

Additional Fibronectin Products

Product Documents for Fibronectin Antibody (2F4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Fibronectin Antibody (2F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fibronectin Antibody (2F4) - BSA Free

Customer Reviews for Fibronectin Antibody (2F4) - BSA Free

There are currently no reviews for this product. Be the first to review Fibronectin Antibody (2F4) - BSA Free and earn rewards!

Have you used Fibronectin Antibody (2F4) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fibronectin Antibody (2F4) - BSA Free

Showing  1 - 1 的 1 FAQ Showing All

    • Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
      A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 
Showing  1 - 1 的 1 FAQ Showing All
View all FAQs for Antibodies