FoxO1/FKHR Antibody (83N7F8) - BSA Free

Name: FoxO1/FKHR Antibody (83N7F8) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-31376
Price: 429 USD
Availability: InStock
Rating: 4 (1 reviews)

Novus Biologicals | Catalog # NBP2-31376

(1)

Citations (8)

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Bovine (91%), Porcine (90%), Squirrel (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 83N7F8

Format

BSA Free

View all FoxO1/FKHR Primary Antibodies »

Product Specifications

Immunogen

FOXO1A

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33662610). Rat reactivity reported in scientific literature (PMID: 31553623).

Localization

Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for FoxO1/FKHR Antibody (83N7F8) - BSA Free

Western Blot: FoxO1/FKHR Antibody (83N7F8)BSA Free [NBP2-31376]

Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: FoxO1/FKHR Antibody (83N7F8)BSA Free [NBP2-31376]

Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Simple Western: FoxO1/FKHR Antibody (83N7F8)BSA Free [NBP2-31376]

Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

View 7 more images

Applications for FoxO1/FKHR Antibody (83N7F8) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.1-2 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:50

Western Blot

0.5-2 ug/ml

Application Notes

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 107 kDa.

Reviewed Applications

Read 1 review rated 4 using NBP2-31376 in the following applications:

Western Blot (1 Review)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxO1/FKHR

The FOX1A transcription factor is a member of forkhead family of transcription factors. It is the main target of insulin signaling. When insulin is present, its activity is suppressed. It regulates metabolic homeostasis in response to oxidative stress. It has several other functions: main regulator of redox balance, control osteoblast numbers, promotes gluconeogenesis (hepatocytes), cell death regulation, promote neural cell death, regulation of adipogenic genes, regulate transcriptional activity of GADD45A, and repair nitric oxide-damaged DNA (beta-cells).

Long Name

Forkhead Box O1

Alternate Names

FKH1, FKHR

Entrez Gene IDs

2308 (Human)

Gene Symbol

FOXO1

UniProt

Q12778 (Human)

Additional FoxO1/FKHR Products

Product Documents for FoxO1/FKHR Antibody (83N7F8) - BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FoxO1/FKHR Antibody (83N7F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Transcription Factors in the Akt Pathway

Citations for FoxO1/FKHR Antibody (83N7F8) - BSA Free

Customer Reviews for FoxO1/FKHR Antibody (83N7F8) - BSA Free (1)

4 out of 5

1 Customer Rating

5 Stars

4 Stars

100%

3 Stars

2 Stars

1 Stars

Have you used FoxO1/FKHR Antibody (83N7F8) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review

Customer Images

Showing 1 - 1 的 1 review Showing All

Filter By:

Name: Anonymous

Application: Western Blot

Sample Tested: Human cancer cell whole cell lysate

Species: Human

Verified Customer | Posted 08/23/2015

FoxO1 expression in human breast cancer cell lines. NBP2-31376