GLI-1 Antibody - BSA Free

Novus Biologicals | Catalog # NB600-600

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Human, Mouse, Porcine, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, ELISA, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLI-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made toward the C-terminus portion of the human GLI-1 protein (between residues 800-850) [Uniprot: P08151]

Reactivity Notes

Canine reactivity reported from a verified customer review.

Localization

Cytoplasmic and Nuclear

Specificity

No reaction occurs with human or mouse Gli2 or Gli3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLI-1 Antibody - BSA Free

Knockdown Validated: GLI-1 Antibody - BSA Free [NB600-600]

Immunohistochemistry: GLI-1 Antibody - BSA Free [NB600-600]

GLI-1-Antibody-Immunohistochemistry-Paraffin-NB600-600-img0005.jpg
Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600]

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600]

Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Analysis of a FFPE mouse brain section using GLI-1 antibody at 1:600 dilution.
Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600]

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600]

Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.
Flow (Intracellular): GLI-1 Antibody - BSA Free [NB600-600]

Flow (Intracellular): GLI-1 Antibody - BSA Free [NB600-600]

Flow (Intracellular): GLI-1 Antibody [NB600-600] - Staining for GLI-1 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-1 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.
GLI-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] -

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] - Paracrine signaling of tumour-derived SHH in CAFs promotes lymphangiogenesis in vivo(A) Growth properties of subcutaneous tumours in mice derived from Caov3 SHH-OE or Caov3 NC cells alone or co-injected with CAFs. Kaplan-Meier plot of time to tumour progression, as evidenced by survival rates over time (n=5). (B) Bright field images of subcutaneous tumours from each group (n=5) 20 days after tumour implantation. (C) Graphical representation of the weights of subcutaneous tumours from each group 20 days after tumour implantation. (D) Immunohistochemical staining for Ki-67, alpha -SMA, Gli-1 & LYVE1 in tumour sections from mice in each group. (E) Graphical representation of the number of LYVE1+ vessels counted from IHC images in panel D. (*P<0.05, **P<0.01, ***P<0.001). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.18621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GLI-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] -

Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo & in vitro(A) Representative images & statistical analyses of migration of LECs in a co-culture invasion system with CAFs stably transfected with shNC or Gli-shRNA lentivirus in the presence of rSHH (1 μg/ml). (B) Representative images & statistical analyses of capillary tube formation of LECs treated with supernatants from cultures of CAFs stably transfected with shNC or Gli-shRNA lentivirus in the presence of rSHH (1 μg/ml). (C) Bright field images & graphical representation of the weights of tumours of subcutaneous tumours from mice (n=5) bearing Caov3 SHH-OE cells co-injected with CAFs stably transfected with shNC or Gli-shRNA lentivirus 20 days after tumour implantation. (D) Growth properties of subcutaneous tumours in mice derived from Caov3 SHH-OE cells co-injected with CAFs stably transfected with shNC or Gli-shRNA lentivirus. Kaplan-Meier plot of time to tumour progression, as evidenced by survival rates over time. (E) IHC staining for Ki-67, alpha -SMA, Gli-1 & LYVE1 in tumour sections from mice in the two groups. (F) Graphical representation of the number of LYVE1+ vessels counted from IHC images in panel E. (**P<0.01, ***P<0.001). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.18621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GLI-1 Antibody - BSA Free

Western Blot: GLI-1 Antibody - BSA Free [NB600-600] -

Comparison of SHH and GLI-1 expression in the normal mammary gland and MGT tissues. A For western blotting, the blots were cut prior to hybridisation with antibodies. SHH (35 kDa) and GLI-1 (100–140 kDa) protein levels in the normal mammary gland and benign and malignant mammary gland tissues. Beta-actin was used as the loading control. B Relative quantitation of SHH and (C) GLI-1 proteins were normalized to that of the control group. The mean and standard error of the mean are used to express quantitative data. (*, **, #: p <.05). SHH, sonic hedgehog; GLI-1, glioma-associated oncogene 1; MGT, mammary gland tumour Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37932728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GLI-1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

1:500 - 1:2000

Flow (Intracellular)

1-2 ug/million cells

Immunoblotting

1 - 2 ug/ml

Immunocytochemistry/ Immunofluorescence

1 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1 - 2 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID:35226587) Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Flow was reported in a customer review. IHC-P reported from a verified customer review. Use in immunoblotting reported in scientific literature (PMID: 28167297). Use in FLOW reported in scientific literature (PMID: 26238488)..

Reviewed Applications

Read 1 review rated 5 using NB600-600 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLI-1

Gli1 is a transcription factor involved in development, patterning and the regulation of cell fate-determination. A down-stream effector of the Hedgehog (HH) signaling pathway, Gli proteins work with other transcriptional activators to up-regulate the expression of Gli-response genes. In addition to its role in embryogenesis, the aberrant activation of the HH/Gli pathway has been identified in numerous forms of cancer, implicating the Gli family of proteins as potential therapeutic targets. In the absence of a stimulus, Gli1 is anchored to the cytoplasm via Cos-2, which is then released and translocates to the nucleus upon activation of the HH pathway. In the nucleus, Gli1 binds DNA via a highly conserved C2-H2 zinc finger domain and acts exclusively as an activator increasing transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin.

Long Name

Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]

Alternate Names

GLI1

Entrez Gene IDs

2735 (Human); 14632 (Mouse)

Gene Symbol

GLI1

UniProt

P08151

Additional GLI-1 Products

Product Documents for GLI-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GLI-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GLI-1 Antibody - BSA Free

Customer Reviews for GLI-1 Antibody - BSA Free (1)

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Customer Images

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  • GLI-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Skin (hair follicle)
    Species: Canine
    Verified Customer | Posted 06/12/2019
    Immunoreactivity for Gli-1 is observed mainly in the isthmus region of the hair follicle.
    Antibody dilution = 1:600. 3, 3'-diaminobenzidine (DAB) chromogen and counterstaining with Mayer’s hematoxylin.
    GLI-1 Antibody - BSA Free NB600-600

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Protocols

View specific protocols for GLI-1 Antibody - BSA Free (NB600-600):


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.


Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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FAQs

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