Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Alexa Fluor® 350-conjugated Antibody

Catalog #: AF1018U Datasheet / COA / SDS

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AF1018U-100UG

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All ERK1 Antibodies

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Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing ERK1 T202/Y204 sites

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ERK1

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.

Long Name

Extracellular Signal-regulated Kinase 1

Entrez Gene IDs

5595 (Human); 26417 (Mouse); 50689 (Rat)

Alternate Names

EC 2.7.11; ERK1; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24