Human p63/TP73L Antibody [Biotin]

R&D Systems | Catalog # BAF1916

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all p63/TP73L Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p63/TP73L
Met40-Cys339
Accession # Q9H3D4

Specificity

Detects p63/TP73L in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human p63/TP73L Antibody [Biotin]

p63/TP73L antibody in Human Prostate by Immunohistochemistry (IHC-P).

p63/TP73L in Human Prostate.

p63/TP73L was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p63/TP73L antibody in SCC-25 Human Cell Line by Immunocytochemistry (ICC).

p63/TP73L in SCC-25 Human Cell Line.

p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human p63/TP73L Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SCC-25 human tongue carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate array

Western Blot

0.1 µg/mL
Sample: Recombinant Human p63/TP73L

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p63/TP73L

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

References

  1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
  2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
  3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.

Long Name

Tumor Protein 63

Alternate Names

EEC3, KET, OFC8, p40, p51, p73L, SHFM4, TP63, TP73L

Entrez Gene IDs

8626 (Human)

Gene Symbol

TP63

UniProt

Q9H3D4

Additional p63/TP73L Products

Product Documents for Human p63/TP73L Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Human p63/TP73L Antibody [Biotin]

For research use only

Citations for Human p63/TP73L Antibody [Biotin]

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Protocols

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