Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry

Cited:

Immunohistochemistry-Frozen, Affinity Purification

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 53-6.7
View all CD8 Primary Antibodies »

Product Specifications

Immunogen

Mouse thymus and spleen cells

Specificity

Detects mouse CD8 alpha. Reacts with alpha chains of mouse CD8 (Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CD8 alpha Biotinylated Antibody

Detection of CD8 alpha antibody in Mouse Splenocytes antibody by Flow Cytometry

Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry

Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated CD8 alpha Monoclonal Antibody (Catalog # BAM116) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.

CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD8 alpha in Mouse Splenocytes.

CD8a was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD8a Biotinylated Monoclonal Antibody (Catalog # BAM116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD8 alpha in Mouse Splenocytes.

CD8a was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8a Biotinylated Monoclonal Antibody (Catalog # BAM116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse CD8 alpha Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma delta TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References

  1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. Hayakawa, K. et al. (1994) Science 263:1131.
  4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  5. Rocha, B. et al. (1992) Immunol. Today 13:449.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
  8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

Additional CD8 Products

Product Documents for Mouse CD8 alpha Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CD8 alpha Biotinylated Antibody

For research use only

Citations for Mouse CD8 alpha Biotinylated Antibody

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Protocols

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