CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma delta TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).
Mouse CD8 Antibody (53-6.7) [Biotin]
R&D Systems | Catalog # BAM116
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry
Cited:
Immunohistochemistry-Frozen, Affinity Purification
Label
Biotin
Antibody Source
Monoclonal Rat IgG2A Clone # 53-6.7
Loading...
Product Specifications
Immunogen
Mouse thymus and spleen cells
Specificity
Detects mouse CD8 alpha. Reacts with alpha chains of mouse CD8 (Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD8 Antibody (53-6.7) [Biotin]
Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry
Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated CD8 alpha Monoclonal Antibody (Catalog # BAM116) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.
CD8 alpha in Mouse Splenocytes.
CD8a was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD8a Biotinylated Monoclonal Antibody (Catalog # BAM116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD8 alpha in Mouse Splenocytes.
CD8a was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8a Biotinylated Monoclonal Antibody (Catalog # BAM116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse CD8 Antibody (53-6.7) [Biotin]
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CD8
References
- Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
- Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
- Hayakawa, K. et al. (1994) Science 263:1131.
- MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
- Rocha, B. et al. (1992) Immunol. Today 13:449.
- Wang, J. and J.R. Klein (1994) Science 265:1860.
- Vermec, D. et al. (1992) J. Exp. Med. 176:47.
- Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
Alternate Names
CD8, CD8A
Entrez Gene IDs
Gene Symbol
CD8A
Additional CD8 Products
Product Documents for Mouse CD8 Antibody (53-6.7) [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CD8 Antibody (53-6.7) [Biotin]
For research use only
Citations for Mouse CD8 Antibody (53-6.7) [Biotin]
Powered by Bioz
Customer Reviews for Mouse CD8 Antibody (53-6.7) [Biotin]
There are currently no reviews for this product. Be the first to review Mouse CD8 Antibody (53-6.7) [Biotin] and earn rewards!
Have you used Mouse CD8 Antibody (53-6.7) [Biotin]?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
