VCAM-1/CD106 Antibody (1.4C3) [HRP]

Novus Biologicals | Catalog # NBP2-33182H

Clone 1.4C3 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

ELISA

Label

HRP

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1.4C3
View all VCAM-1/CD106 Primary Antibodies »

Product Specifications

Immunogen

Stimulated human umbilical vein endothelial cells (HUVEC)

Localization

Cell surface

Marker

Activated Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Scientific Data Images for VCAM-1/CD106 Antibody (1.4C3) [HRP]

Western Blot: VCAM-1/CD106 Antibody (1.4C3) [HRP] [NBP2-33182H] - analysis of VCAM-1 in HDMVE cell lysate using anti-VCAM-1 antibody. Image from verified customer review.
ELISA: VCAM-1/CD106 Antibody (1.4C3) [HRP] [NBP2-33182H] - In-cell ELISA performed with HDMVE cells using HRP conjugated VCAM-1 antibody. Image from verified customer review.

Applications for VCAM-1/CD106 Antibody (1.4C3) [HRP]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA reported by a customer review and from a citation (PMID:27799312). WB reported by a customer review.

Reviewed Applications

Read 2 reviews rated 5 using NBP2-33182H in the following applications:

Spectra Viewer

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Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: VCAM-1/CD106

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (1.4C3) [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VCAM-1/CD106 Antibody (1.4C3) [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VCAM-1/CD106 Antibody (1.4C3) [HRP]

Customer Reviews for VCAM-1/CD106 Antibody (1.4C3) [HRP] (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 的 2 reviews Showing All
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
    Species: Human
    Verified Customer | Posted 11/10/2015
    Western blot using HDMVEC cell lysate
    VCAM-1/CD106 Antibody (1.4C3) [HRP] NBP2-33182H
  • Name: Anonymous
    Application: ELISA
    Sample Tested: Human Dermal Microvascular Endothelial cells - fixed cells used in In-cell ELISA
    Species: Human
    Verified Customer | Posted 11/10/2015
    In-cell ELISA with HDMVECs
    VCAM-1/CD106 Antibody (1.4C3) [HRP] NBP2-33182H

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