Vimentin Antibody (VM452) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-33060

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Porcine, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative)

Applications

Knockout Validated, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # VM452

Format

Azide and BSA Free
View all Vimentin Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human vimentin protein (Uniprot: P08670)

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Cytoplasmic

Marker

Mesenchymal Cell Marker

Specificity

This monoclonal antibody reacts with a 58kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of Vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-Vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-Vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain Vimentin, e.g. hepatoma and seminoma. Anti-Vimentin is also useful as a tissue process control reagent.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

53.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29433).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Vimentin Antibody (VM452) - Azide and BSA Free

Knockout Validated: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060]

Western Blot: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060]

Western Blot: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-33060) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.
Western Blot: Vimentin Antibody (VM452)Azide and BSA Free [NBP2-33060]

Western Blot: Vimentin Antibody (VM452)Azide and BSA Free [NBP2-33060]

Western Blot: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).
Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060]

Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060]

Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).
Flow Cytometry: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060]

Flow Cytometry: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060]

Flow Cytometry: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).
Western Blot: Vimentin Antibody (VM452)Azide and BSA Free [NBP2-33060]

Western Blot: Vimentin Antibody (VM452)Azide and BSA Free [NBP2-33060]

Western Blot: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).
Protein Array: Vimentin Antibody (VM452)Azide and BSA Free [NBP2-33060]

Protein Array: Vimentin Antibody (VM452)Azide and BSA Free [NBP2-33060]

Protein Array: Vimentin Antibody (VM452) - Azide and BSA Free [NBP2-33060] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Vimentin Antibody (VM452) - Azide and BSA Free

Application
Recommended Usage

Western Blot

0.5 - 2.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Vimentin

Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

Alternate Names

VIM

Gene Symbol

VIM

Additional Vimentin Products

Product Documents for Vimentin Antibody (VM452) - Azide and BSA Free

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Product Specific Notices for Vimentin Antibody (VM452) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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