Tumors recruit immunosuppressive immune cells which create a microenvironment that inhibits anti-tumor responses and enables tumor growth. Monitoring the spatial and temporal dynamics of tumor infiltrating lymphocytes (TILs) and other immune cells in the tumor microenvironment is crucial for the development of more effective immune checkpoint blockade strategies. R&D Systems offers a wide selection of flow cytometry antibodies and kits for phenotyping immune cells and cell selection kits to isolate them for downstream studies.
Flow Cytometry Antibodies for Immune Cell Markers
Click the buttons below to view select markers commonly used to identify each cell type. The lists of markers are general and it is recognized that there are different subtypes of many of the cell types below (e.g. macrophages and NKT cells) with opposing functions in the anti-tumor immune response. Molecules are linked directly to flow cytometry antibodies making it easy to find what you need.
Multi-color Flow Cytometry Kits
Identify cell subsets with our rapid and efficient multi-color flow cytometry kits that simultaneously stain multiple targets in a single step. Our kits include fluorochrome-conjugated antibodies, isotype controls for each antibody, and all necessary buffers.
|Th17 Multi-Color Flow Cytometry Kit. Human PBMCs were stimulated overnight with PMA (50 ng/mL; Catalog # 1201), ionomycin (200 ng/mL), IL-23 (10 ng/mL; Catalog # 1290-IL), and LPS (500 ng/mL), followed by 5 hours re-stimulation with PMA/ionomycin and 3 mM monensin. Cells were stained with the human Th17 Multi-color Flow Cytometry Kit (Catalog # FMC007). Dot plots show IL-17+ cells in relation to CD3+, IL-23 R+, or IL-22+ populations from activated PBMCs. Quadrants were set based on isotype controls.
MagCellect™ Cell Selection Kits
Our cell selection kits utilize MagCellect technology based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (i.e. superparamagnetic). This feature protects purified cell populations against magnetic forces which may affect cell viability and interfere with downstream applications.
Immune Cell Markers