Immune Checkpoint Blockade: Immune Cell Phenotyping and Isolation

Tumors recruit immunosuppressive immune cells which create a microenvironment that inhibits anti-tumor responses and enables tumor growth. Monitoring the spatial and temporal dynamics of tumor infiltrating lymphocytes (TILs) and other immune cells in the tumor microenvironment is crucial for the development of more effective immune checkpoint blockade strategies. R&D Systems offers a wide selection of flow cytometry antibodies and kits for phenotyping immune cells and cell selection kits to isolate them for downstream studies.

Immune Checkpoint Blockade Homepage

Flow Cytometry Antibodies for Immune Cell Markers

Click the buttons below to view select markers commonly used to identify each cell type. The lists of markers are general and it is recognized that there are different subtypes of many of the cell types below (e.g. macrophages and NKT cells) with opposing functions in the anti-tumor immune response. Molecules are linked directly to flow cytometry antibodies making it easy to find what you need.

B Cell Markers T Cell Markers Th17 Cell Markers Regulatory T Cell Markers NK Cell Markers
NKT Cell Markers Monocyte Markers Macrophage Markers Dendritic Cell Markers MDSC Markers

Multi-color Flow Cytometry Kits

Identify cell subsets with our rapid and efficient multi-color flow cytometry kits that simultaneously stain multiple targets in a single step. Our kits include fluorochrome-conjugated antibodies, isotype controls for each antibody, and all necessary buffers.

Regulatory T Cell Kits Th17 Cell Kits Dendritic Cell Kits View All Flow Cytometry Kits
Th17 Multi-Color Flow Cytometry Kit. Human PBMCs were stimulated overnight with PMA (50 ng/mL; Catalog # 1201), ionomycin (200 ng/mL), IL-23 (10 ng/mL; Catalog # 1290-IL), and LPS (500 ng/mL), followed by 5 hours re-stimulation with PMA/ionomycin and 3 mM monensin. Cells were stained with the human Th17 Multi-color Flow Cytometry Kit (Catalog # FMC007). Dot plots show IL-17+ cells in relation to CD3+, IL-23 R+, or IL-22+ populations from activated PBMCs. Quadrants were set based on isotype controls.

MagCellect Cell Selection Kits

Our cell selection kits utilize MagCellect technology based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (i.e. superparamagnetic). This feature protects purified cell populations against magnetic forces which may affect cell viability and interfere with downstream applications. 

CD3+ T Cells CD4+ T Cells CD8+ T Cells Regulatory T Cells
NK Cells CD14+ Monocytes/Macrophages B Cells View All Cell Selection Kits

Immune Cell Markers

B Cell Markers
Human Mouse
CD19 CD19
MS4A1/CD20

Siglec-2/CD22

 

B220/CD45R

T Cell Markers
Human Mouse
CD3 CD3
CD4 CD4
CD8 CD8
Th17 Cell Markers
Human Mouse

CD4

CD4

IL-17A

IL-17A

ROR gamma t

ROR gamma t

Regulatory T Cell Markers
Human Mouse

CD4

CD4

CD25/IL-2 R alpha

CD25/IL-2 R alpha

FoxP3

FoxP3

GITR/TNFRSF18

GITR/TNFRSF18

IL-7 R alpha/CD127dim

 
NK Cell Markers
Human Mouse

CD3

CD3

NCAM-1/CD56

CD27/TNFRSF7

Fc gamma RIII (CD16)

CD161/NK1.1

IFN-gamma

IFN-gamma

 

Integrin alpha 2/

CD49b

 

Integrin alpha M/

CD11b

 

NKp46/NCR1

NKT Cell Markers
Human Mouse

CD3

CD3

V alpha 24

CD1d tetramer

V beta 11

TCR beta

CD56

CD161/NK1.1

CD1d tetramer

CD44

Fc gamma RIII (CD16)

V alpha 14

Monocyte Markers
Human Mouse

CD14

Integrin alpha M/

CD11b

Fc gamma RIII (CD16)

Ly-6C

HLA-DR

F4/80/EMR1

 

Gr-1/Ly-6G

 

M-CSF R

Macrophage Markers
Human Mouse

CD68/SR-D1

F4/80/EMR1

CD14

Integrin alpha M/

CD11b

CD163

MMR/CD206

MMR/CD206

CD11c

Stabilin-1

CD68/SR-D1

HLA-DR

Arginase1/

ARG1

SR-AI/MSR

CD45

 

MHC-II

Dendritic Cell Markers
Human Mouse

HLA-DR

CD11c

B7-2/CD86

MHC-II

B7-1/CD80

B7-2/CD86

CD11c

Integrin alpha M/

CD11b

CD83

B7-1/CD80

CD14

Integrin alpha E/

CD103

CD1a

CD8 alpha

CD40/TNFRSF5

 

DC-SIGN/CD209

 

CD1c/BDCA-1

 
MDSC Markers
Human Mouse

Integrin alpha M/

CD11b

Integrin alpha M/

CD11b

CD14

Gr-1/Ly-6G

Siglec-3/CD33

Ly-6C

CD15/Lewis X

 

HLA-DR-