alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-34522

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Chicken, Feline, Goat, Guinea Pig, Monkey, Rabbit, Sheep

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A4/asm-1

Format

Azide and BSA Free
View all alpha-Smooth Muscle Actin Primary Antibodies »

Product Specifications

Immunogen

Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) is made to the N-Terminal decapeptide of Alpha smooth muscle isoform of actin and conjugated to KLH.

Reactivity Notes

Feline reactivity reported from a verified customer review.

Localization

Cytoplasmic

Marker

Leiomyosarcoma Marker

Specificity

Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Western Blot Detection of alpha-Smooth Muscle Actin in Human Heart Tissue and iBJ6 hiPSCs

Western Blot Detection of alpha-Smooth Muscle Actin in Human Heart Tissue and iBJ6 hiPSCs

Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 ug/mL of Mouse Anti-alpha-Smooth Muscle Actin Monoclonal Antibody (Catalog # NBP2-33006) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). The observed molecular weight was detected for alpha Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions. Image using the standard format of this product.
Immunohistological Staining of alpha-Smooth Muscle Actin in Paraffin Embedded Cancer Tissues

Immunohistological Staining of alpha-Smooth Muscle Actin in Paraffin Embedded Cancer Tissues

M2 macrophage-related protein expression and the co-location of M2-macrophages and GC-MSCs within tumor tissues. Representative immunofluorescence images of the co-location of GC-MSCs (alpha-Smooth Muscle Actin; green) and M2-macrophages (CD204; red) in gastric cancer tissues detected by confocal microscopy. scale bar, 100 um. **P < 0.01 vs. gastric cancer tissue. T: tumor; N: non-cancerous tissues. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41419-019-2131-y), licensed under a CC-BY license. Image using the standard format of this product.
Flow Cytometry Analysis of alpha-Smooth Muscle Actin in Colorectal Tumor Cells using Allophycocyanin Conjugated Antibody

Flow Cytometry Analysis of alpha-Smooth Muscle Actin in Colorectal Tumor Cells using Allophycocyanin Conjugated Antibody

alpha-Smooth Muscle Actin expression in colorectal tumor cells. Using Allophycocyanin conjugated version of antibody (NBP2-34522APC). Image from verified customer review.
Immunohistological Staining of alpha-Smooth Muscle Actin in Frozen Human Tonsil

Immunohistological Staining of alpha-Smooth Muscle Actin in Frozen Human Tonsil

Human tonsil frozen tissue section stained for alpha-Smooth Muscle Actin (red) with the Janelia Fluor 549 conjugate of the Azide and BSA-Free version of alpha-Smooth Muscle Actin Antibody (1A4/asm-1), and counterstained with DAPI (blue). Image from verified customer review.
Immunohistological Detection of alpha-Smooth Muscle Actin in Paraffin Embedded Human Leiomyosarcoma

Immunohistological Detection of alpha-Smooth Muscle Actin in Paraffin Embedded Human Leiomyosarcoma

Paraffin-embedded human Leiomyosarcoma statined with the Azide and BSA-Free alpha-Smooth Muscle Actin Antibody (1A4/asm-1).
Immunohistological Staining of alpha-Smooth Muscle Actin in Frozen Canine Brain/Spinal Cord

Immunohistological Staining of alpha-Smooth Muscle Actin in Frozen Canine Brain/Spinal Cord

Canine brain/spinal cord section. Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) staining in red. Endothelial cell marker in green. DAPI in blue. Alexa Fluor conjugated antibodies used for secondary antibodies. IHC image submitted by a verified customer review. Image using the standard format of this product.
Immunohistological Imaging of alpha-Smooth Muscle Actin in Frozen Feline Tissue

Immunohistological Imaging of alpha-Smooth Muscle Actin in Frozen Feline Tissue

Feline tissue stained with alpha-Smooth Muscle Actin Antibody at dilution of 1:600. Vascular wall was stained. IHC image submitted by a verified customer review. Image using the standard format of this product.
Flow Cytometry of HeLa Cells Stained with Phycoerythrin Conjugated alpha-Smooth Muscle Actin Antibody

Flow Cytometry of HeLa Cells Stained with Phycoerythrin Conjugated alpha-Smooth Muscle Actin Antibody

An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Flow Cytometry of HeLa Cells Stained with Alexa Fluor 647 Conjugated alpha-Smooth Muscle Actin Antibody

Flow Cytometry of HeLa Cells Stained with Alexa Fluor 647 Conjugated alpha-Smooth Muscle Actin Antibody

An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry of HeLa Cells Stained with Allophycocyanin Conjugated alpha-Smooth Muscle Actin Antibody

Flow Cytometry of HeLa Cells Stained with Allophycocyanin Conjugated alpha-Smooth Muscle Actin Antibody

An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Flow Cytometry of HeLa Cells Stained with PerCP Conjugated alpha-Smooth Muscle Actin Antibody

Flow Cytometry of HeLa Cells Stained with PerCP Conjugated alpha-Smooth Muscle Actin Antibody

An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.
Flow Cytometry of HeLa Cells Stained with Alexa Fluor 700 Conjugated alpha-Smooth Muscle Actin Antibody

Flow Cytometry of HeLa Cells Stained with Alexa Fluor 700 Conjugated alpha-Smooth Muscle Actin Antibody

An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
Flow Cytometry of HeLa Cells Stained with Janelia Fluor 549 Conjugated alpha-Smooth Muscle Actin Antibody

Flow Cytometry of HeLa Cells Stained with Janelia Fluor 549 Conjugated alpha-Smooth Muscle Actin Antibody

An intracellular stain was performed on HeLa cells with alpha Smooth Muscle Actin (1A4/asm-1) Antibody NBP2-34522JF549 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Janelia Fluor 549.
Electropherogram Representation of alpha-Smooth Muscle Actin Detection in Aorta and Uterus Lysates by Simple Western

Electropherogram Representation of alpha-Smooth Muscle Actin Detection in Aorta and Uterus Lysates by Simple Western

Electropherogram image of the corresponding Simple Western lane. alpha-Smooth Muscle Actin antibody was used at 10 ug/ml dilution of h. Aorta and h. Uterus lysates(s) respectively. Image using the standard format of this product.
Simple Western Analysis of alpha-Smooth Muscle Actin in Aorta Lysate

Simple Western Analysis of alpha-Smooth Muscle Actin in Aorta Lysate

Simple Western lane view shows a specific band for an observed molecular weight at ~45 kDa for alpha-Smooth Muscle Actin in 0.2 mg/ml of h. Aorta lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image using the standard format of this product.
alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free [NBP2-34522]

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - Analysis of alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [Alexa Fluor® 647] (Catalog Number: NBP2-34522AF647) using two lung cancer cell lines to acquire the subcutaneous tumors. MMT status of macrophages was then analyzed. Image from a verified customer review.
alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free [NBP2-34522]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free [NBP2-34522] - aSMA stained over night in human FFPE tonsil section ((Catalog # NBP2-33006AF750)). Heat mediated antigen retrieval at pH 9. 1:50 Dilution in 3% BSA, incubation over night at 4 °C. Image from a verified customer review.
alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free [NBP2-34522]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free [NBP2-34522] - aSMA stained over night in human FFPE tonsil section ((Catalog # NBP2-33006AF750)). Heat mediated antigen retrieval at pH 9. 1:50 Dilution in 3% BSA, incubation over night at 4 °C. Image from a verified customer review.

Applications for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Application
Recommended Usage

ELISA

1-5ug/ml for coating

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Frozen

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Immunoprecipitation

0.5-1ug/500ug protein lysate
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID:34276989).

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2.El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G.,... Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M.,... Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Customer Reviews for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free and earn rewards!

Have you used alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?

    A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

  • Q: Which antibodies would you recommend for the best performance in IHC?

    A: NB300-978, NBP1-30894, NBP2-22120, NBP2-32808, NBP2-33006, and NBP2-34522 have the broadest IHC applications, as they additionally list IHC-Paraffin and IHC-Frozen. To narrow the list down further, we would recommend using either NB300-978 or NBP2-33006 since these products have the most ratings and cited publications for use in IHC methods. NB300-978 has IHC-Free-Floating listed as an application as well.

  • Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?

    A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

  • Q: Which antibodies would you recommend for the best performance in IHC?

    A: NB300-978, NBP1-30894, NBP2-22120, NBP2-32808, NBP2-33006, and NBP2-34522 have the broadest IHC applications, as they additionally list IHC-Paraffin and IHC-Frozen. To narrow the list down further, we would recommend using either NB300-978 or NBP2-33006 since these products have the most ratings and cited publications for use in IHC methods. NB300-978 has IHC-Free-Floating listed as an application as well.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies