Anti- Ipilimumab (Anti-Idiotype) Antibody

Catalog # Availability Size / Price Qty
MAB10064-100
MAB10064-SP
Detection  of Ipilimumab by Western Blot.
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Anti- Ipilimumab (Anti-Idiotype) Antibody Summary

Specificity
Detects Ipilimumab in direct ELISAs and Western blots. In Western blots, no cross-reactivity with Cetuximab, Brentuximab, Teprotumumab, and Aducanumab is observed.
Source
Monoclonal Mouse IgG2A Clone # 1001345
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Full length Ipilimumab
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Ipilimumab by Western Blot. View Larger

Detection of Ipilimumab by Western Blot. Western blot shows lysates of Cetuximab, Brentuximab, Teprotumumab, Aducanumab, and Ipilimumab. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Anti- Ipilimumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10064) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ipilimumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ipilimumab

The anti-Ipilimumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Ipilimumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names
Ipilimumab

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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