Anti- Ipilimumab (Anti-Idiotype) Antibody

Catalog # Availability Size / Price Qty
Detection of Ipilimumab by Western Blot.
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Product Details
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Anti- Ipilimumab (Anti-Idiotype) Antibody Summary

Detects Ipilimumab in direct ELISAs and Western blots. In Western blots, no cross-reactivity with Cetuximab, Brentuximab, Teprotumumab, and Aducanumab is observed.
Monoclonal Mouse IgG2A Clone # 1001345
Protein A or G purified from hybridoma culture supernatant
Full length Ipilimumab
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Ipilimumab antibody by Western Blot. View Larger

Detection of Ipilimumab by Western Blot. Western blot shows lysates of Cetuximab, Brentuximab, Teprotumumab, Aducanumab, and Ipilimumab. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Anti- Ipilimumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10064) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ipilimumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ipilimumab

The anti-Ipilimumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Ipilimumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names

Product Datasheets

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  1. What is an anti-idiotype antibody?

    • An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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