B-Raf Antibody (BRAF-V600E/13276R)

Name: B-Raf Antibody (BRAF-V600E/13276R)
Brand: Novus Biologicals
SKU: NBP3-48992
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-48992

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BRAF-V600E/13276R expressed in CHO

View all B-Raf Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment corresponding to human BRAF V600E mutant protein (Uniprot: P15056)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for B-Raf Antibody (BRAF-V600E/13276R)

Immunohistochemistry-Paraffin: B-Raf Antibody (BRAF-V600E/13276R) [NBP3-48992] -

Formalin-fixed, paraffin-embedded human melanoma stained with B-Raf Antibody (BRAF-V600E/13276R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for B-Raf Antibody (BRAF-V600E/13276R)

Application

Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody (BRAF-V600E/13276R)

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Certificate of Analysis

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Product Specific Notices for B-Raf Antibody (BRAF-V600E/13276R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.