B-Raf Antibody

Name: B-Raf Antibody
Brand: Novus Biologicals
SKU: NBP2-15531
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-15531

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all B-Raf Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human B-Raf. The exact sequence is proprietary.

Reactivity Notes

Chicken (89%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit B-Raf Antibody (NBP2-15531) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for B-Raf Antibody

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-15531]

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-15531] - Paraffin-embedded mouse testis. B-Raf antibody [N2C1], Internal dilution: 1:500.

Western Blot: B-Raf Antibody [NBP2-15531]

Western Blot: B-Raf Antibody [NBP2-15531] - A. 30 ug PC-12 whole cell extract 7.5 % SDS-PAGE B-Raf antibody [N2C1], Internal dilution: 1:5000

Western Blot: B-Raf Antibody [NBP2-15531]

Western Blot: B-Raf Antibody [NBP2-15531] - Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal diluted at 1:5000.

Immunoprecipitation: B-Raf Antibody [NBP2-15531]

Immunoprecipitation: B-Raf Antibody [NBP2-15531] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 ug of B-Raf antibody [N2C1], Internal 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-15531] -

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-15531] - B-Raf antibody [N2C1], Internal detects B-Raf protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
B-Raf stained by B-Raf antibody [N2C1], Internal (NBP2-15531) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: B-Raf Antibody [NBP2-15531] -

Western Blot: B-Raf Antibody [NBP2-15531] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (NBP2-15531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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Applications for B-Raf Antibody

Application

Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: B-Raf

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq]

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

UniProt

P15056

Additional B-Raf Products

Product Documents for B-Raf Antibody

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Certificate of Analysis

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Product Specific Notices for B-Raf Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.