beta-Catenin Antibody (12F7) [FITC]

Novus Biologicals | Catalog # NBP1-54467F

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Duck, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 12F7
View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

Recombinant chicken beta Catenin fused to maltose binding protein. [UniProt# O42486]

Reactivity Notes

Use in Duck reported in scientific literature (PMID:32692763).

Localization

Nucleus, Cytoplasm, Cytoskeleton, Adherens junction, Centrosome, Spindle pole.

Marker

Epithelial Cell Marker, Adherens Junction Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for beta-Catenin Antibody (12F7) [FITC]

Immunohistochemistry-Frozen: beta-Catenin Antibody (12F7) [FITC] [NBP1-54467F] - Beta-catening (green) was detected in human skin (psoriasis) using beta-catenin-FITC antibody (1:40) in PBS for 1 hour. Nuclei were stained with Dapi (blue). Image from verified customer review.

Applications for beta-Catenin Antibody (12F7) [FITC]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-54467F in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (12F7) [FITC]

Certificate of Analysis

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Product Specific Notices for beta-Catenin Antibody (12F7) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta-Catenin Antibody (12F7) [FITC]

Customer Reviews for beta-Catenin Antibody (12F7) [FITC] (1)

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Customer Images

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  • beta-Catenin Antibody (12F7) [FITC]
    Name: Christian Ostalecki
    Application: Immunohistochemistry-Frozen
    Sample Tested: Human skin
    Species: Human
    Verified Customer | Posted 08/06/2018
    Immunohistochemistry-Frozen: beta-catening (green) was detected in human skin (psoriasis) using beta-catenin-FITC antibody (NBP1-54467F) with a concentration of 1:40 in PBS for 1 hour. Nuclei were stained with Dapi (blue).
    beta-Catenin Antibody (12F7) [FITC] NBP1-54467F

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