beta-Catenin Antibody (12F7) - Azide and BSA Free

Name: beta-Catenin Antibody (12F7) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-80596
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-80596

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Duck, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 12F7

Format

Azide and BSA Free

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

Recombinant chicken beta Catenin fused to maltose binding protein. [UniProt# O42486]

Localization

Nucleus, Cytoplasm, Cytoskeleton, Adherens junction, Centrosome, Spindle pole.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Catenin Antibody (12F7) - Azide and BSA Free

Western Blot: beta-Catenin Antibody (12F7)Azide and BSA Free [NBP2-80596]

Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.

Immunocytochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596]

Immunocytochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain. Image from the standard format of this antibody.

Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596]

Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta Catenin in mouse intestine using DAB with hematoxylin counterstain. Image from the standard format of this antibody.

Flow Cytometry: beta-Catenin Antibody (12F7)Azide and BSA Free [NBP2-80596]

Flow Cytometry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals). Image from the standard format of this antibody.

Western Blot: beta-Catenin Antibody (12F7)Azide and BSA Free [NBP2-80596]

Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Activity of Wnt/beta-catenin pathway in the embryonic chick lung. Western blot analysis of active and total beta-catenin in stage b1, b2 and b3 lungs, and stage 24 limb (as positive control). Control loading was performed using beta-tubulin (55 kDa). Total and active beta-catenin correspond to 92 kDa. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0112388), licensed under a CC-BY license. Image from the standard format of this antibody.

Western Blot: beta-Catenin Antibody (12F7)Azide and BSA Free [NBP2-80596]

Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467. Image from the standard format of this antibody.

Western Blot: beta-Catenin Antibody (12F7)Azide and BSA Free [NBP2-80596]

Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta- Catenin in embryonic lung (lane 1) and embryonic limb (lane 2) lysates using anti-beta- Catenin antibody. Each lane was loaded with 5ug of protein sample. Image from verified customer review. Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue). Image from the standard format of this antibody.

Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596]

Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Beta-catening (green) was detected in human skin (psoriasis) using beta-catenin-FITC antibody (1:40) in PBS for 1 hour. Nuclei were stained with Dapi (blue). Image from verified customer review. Image using the FITC format of this antibody. Image from the

Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596]

Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - IF beta-Catenin staining of mouse brain tissue. This image submitted by a verified customer review. Image from the standard format of this antibody.

Simple Western: beta-Catenin Antibody (12F7)Azide and BSA Free [NBP2-80596]

Simple Western: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Simple Western lane view shows a specific band for Beta- Catenin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

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Applications for beta-Catenin Antibody (12F7) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:100-1:200

Immunohistochemistry-Paraffin

1:100-1:200

Immunoprecipitation

1:10-1:500

Simple Western

1:100

Western Blot

1:1000

Application Notes

This beta Catenin (12F7) antibody is useful for IHC-P sections, ICC/IF, IP and WB, where a band can be seen at approx. 92 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta-Catenin

The beta-catenin protein functions in both regulation and coordination of cell-cell adhesion and gene transcription. In humans, beta-catenin the CTNNB1 gene. beta-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-catenin, also known as plakoglobin. Beta-catenin is widely expressed in many tissues. In cardiac muscle, beta-catenin localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocytes.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (12F7) - Azide and BSA Free

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Product Specific Notices for beta-Catenin Antibody (12F7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.