beta-Catenin Antibody (6A3P2)

Name: beta-Catenin Antibody (6A3P2)
Brand: Novus Biologicals
SKU: NBP3-15842
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15842

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Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6A3P2 expressed in HEK293

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (P35222). MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Catenin Antibody (6A3P2)

Western Blot: beta-Catenin Antibody (6A3P2) [NBP3-15842]

Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western Blot: beta-Catenin Antibody (6A3P2) [NBP3-15842]

Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western Blot: beta-Catenin Antibody (6A3P2) [NBP3-15842]

Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts from normal (control) and beta-Catenin knockout (KO) 293T cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunoprecipitation: beta-Catenin Antibody (6A3P2) [NBP3-15842]

Immunoprecipitation: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug beta-Catenin antibody (NBP3-15842). Western blot was performed from the immunoprecipitate using beta-Catenin (NBP3-15842) at a dilition of 1:1000.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from NIH/3T3 cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from wild type(WT) and beta-Catenin knockout (KO) 293T(KO) cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human solitary fibrous tumor tissue using [KO Validated] beta-Catenin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human prostate cancer tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] -

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

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Applications for beta-Catenin Antibody (6A3P2)

Application

Recommended Usage

Chromatin Immunoprecipitation

2μg antibody for 5μg-15μg of Chromatin

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

0.5μg-4μg antibody for 400μg-600μg extracts of whole cells

Western Blot

1:4000 - 1:20000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (6A3P2)

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Certificate of Analysis

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Product Specific Notices for beta-Catenin Antibody (6A3P2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.