beta-Catenin Antibody - BSA Free

Name: beta-Catenin Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-89989
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-89989

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Fish

Cited:

Fish

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAH

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 25502575).

Marker

Epithelial Cell Marker, Adherens Junction Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta-Catenin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-89989]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-89989] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989]

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining in human cervix, uterine and skeletal muscle tissues. Corresponding CTNNB1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989]

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human cervix, uterine shows moderate membranous positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989]

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human skeletal muscle shows weak positivity in myocytes.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989]

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human prostate shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989]

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human duodenum shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989]

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.

Western Blot: beta-Catenin Antibody - BSA Free [NBP1-89989]

Analysis in human cell line SW480.

Western Blot: beta-Catenin Antibody - BSA Free [NBP1-89989]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Western Blot: beta-Catenin Antibody [NBP1-89989]

beta-Catenin-Antibody-Western-Blot-NBP1-89989-img0026.jpg

Western Blot: beta-Catenin Antibody - BSA Free [NBP1-89989] -

Omegaven® inhibits epithelial to mesenchymal transition induced by TGF beta 1.Human liver epithelial cell line THLE-3 was incubated in presence or absence of lipid emulsions Omegaven® 10%, Lipofundin MCT/LCT® 20%, ClinOleic® 20% or SMOFlipid® 20% at different dilutions, for 30 min followed by TGF beta 1 5 ng/mL stimulation for additional 72 hours (A and B) or 25 min (C). (A) Expression of mRNA of ZO-1 and (B) E-cadherin. (C) Phosphorylation of Samd3, ERK1/2 and Akt and nuclear expression of beta -catenin. Representative western blot are showed and quantified in graphic bars. Results are expressed as means +/- SEM of six independent experiments. *p<0.05 related to the control group. #p<0.05 related to the stimulus. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25502575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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Applications for beta-Catenin Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Entrez Gene IDs

1499 (Human)

Gene Symbol

CTNNB1

UniProt

P35222

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Certificate of Analysis

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Product Specific Notices for beta-Catenin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.