BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80597

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3H2-E8

Format

Azide and BSA Free
View all BLIMP1/PRDM1 Primary Antibodies »

Product Specifications

Immunogen

A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]

Reactivity Notes

Porcine usage reported in customer reviews

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8)Azide and BSA Free [NBP2-80597]

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8)Azide and BSA Free [NBP2-80597]

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-BLIMP1/PRDM1 (3H2-E8) conjugated to DyLight 550 [NB600-235R] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Immunohistochemistry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Immunohistochemistry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) and Counterstained by hematoxylin; labeling is predominantly in germinal centers.
Images may not be copied
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ) Image from the standard format of this antibody.
Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8)Azide and BSA Free [NBP2-80597]

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8)Azide and BSA Free [NBP2-80597]

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BLIMP1 (3H2-E8) at 10 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY. Image from the standard format of this antibody.
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8)Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8)Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 [3H2-E8] Antibody NB600-235C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 650.
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - An intracellular stain was performed on U266 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image from the standard format of this antibody.
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ). Image from the standard format of this antibody.
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597]

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free [NBP2-80597] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ). Image from the standard format of this antibody.

Applications for BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1 ug. Use reported in scientific literature

ELISA

1:100-1:2000

Flow Cytometry

2 10 6/ml

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:1000
Application Notes
By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BLIMP1/PRDM1

BLIMP-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor which primarily controls the terminal differentiation of mature B cells to plasma cells. BLIMP-1 is also involved in macrophage and T-cell population homeostasis/differentiation. It is also called positive regulatory domain I-binding factor-1 (PRDI-BF1) or PR (PRDI-BF1-RIZ) domain zinc finger protein 1 (PRDM1) and its first human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-beta promoter leading to inhibition of virus-mediated IFN-beta production. BLIMP-1 contains N-terminal PR/SET domain and five C2H2 zinc fingers located near its C-terminus that mediate DNA binding, nuclear import and recruitment of histone modifying enzymes, and these activities enable BLIMP-1 to control cell-fate decisions in the embryo and govern tissue homeostasis in multiple cell types in the adult organism. BLIMP-1 recruits chromatin-modifying enzymes including HDACs and methyltransferases, and some of its target genes include c-Myc, CIITA, Pax5, Spi-B, and Id3. BLIMP-1 sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Deletion mutation of BLIMP-1 is frequently observed in several tumor types including lymphoid malignancies, and has been proposed to be a candidate for tumor supression research.

Long Name

B Lymphocyte Induced Maturation Protein

Alternate Names

PRDM1, ZNFPR1A1

Gene Symbol

PRDM1

Additional BLIMP1/PRDM1 Products

Product Documents for BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BLIMP1/PRDM1 Antibody (3H2-E8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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