BMP-4 Antibody (3C11H8) - BSA Free

Name: BMP-4 Antibody (3C11H8) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-52423
Price: 139 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-52423

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 3C11H8

Format

BSA Free

View all BMP-4 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human BMP-4 (AA: 277-408) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMP-4 Antibody (3C11H8) - BSA Free

Western Blot: BMP-4 Antibody (3C11H8)BSA Free [NBP2-52423]

Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mAb against human BMP4 (AA: 277-408) recombinant protein. (Expected MW is 41 kDa).

Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11H8) - BSA Free [NBP2-52423]

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis of Hela cells using BMP-4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western Blot: BMP-4 Antibody (3C11H8)BSA Free [NBP2-52423]

Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: BMP-4 Antibody (3C11H8)BSA Free [NBP2-52423]

Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mouse mAb against A549 (1) and C6 (2) cell lysate.

Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11H8) - BSA Free [NBP2-52423]

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11H8) [NBP2-52423] - Immunofluorescence analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

ELISA: BMP-4 Antibody (3C11H8) - BSA Free [NBP2-52423]

ELISA: BMP-4 Antibody (3C11H8) [NBP2-52423] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

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Applications for BMP-4 Antibody (3C11H8) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000

Application Notes

This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMP-4

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Gene Symbol

BMP4

Additional BMP-4 Products

Product Documents for BMP-4 Antibody (3C11H8) - BSA Free

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Certificate of Analysis

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Product Specific Notices for BMP-4 Antibody (3C11H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.