BMP-4 Antibody (7Q2U7)

Name: BMP-4 Antibody (7Q2U7)
Brand: Novus Biologicals
SKU: NBP3-15376
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15376

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7Q2U7 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 309-408 of human BMP-4 (P12644). RRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BMP-4 Antibody (7Q2U7)

Western Blot: BMP-4 Antibody (7Q2U7) [NBP3-15376]

Western Blot: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Analysis of extracts of various cell lines, using BMP-4 Rabbit mAb (NBP3-15376) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (7Q2U7) [NBP3-15376]

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Immunofluorescence analysis of HeLa cells using BMP-4 Rabbit mAb (NBP3-15376) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: BMP-4 Antibody (7Q2U7) [NBP3-15376]

Immunohistochemistry-Paraffin: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Human colon using BMP-4 Rabbit mAb (NBP3-15376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (7Q2U7) [BMP-4] -

Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (7Q2U7) [BMP-4] - Confocal imaging of HeLa cells using BMP-4 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

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Applications for BMP-4 Antibody (7Q2U7)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BMP-4

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Gene Symbol

BMP4

Additional BMP-4 Products

Product Documents for BMP-4 Antibody (7Q2U7)

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Certificate of Analysis

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Product Specific Notices for BMP-4 Antibody (7Q2U7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.