Detects bovine FGF acidic in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize both bovine FGF acidic and recombinant human FGF acidic. In direct ELISAs, no cross‑reactivity with __________________ is observed. Neutralizes the biological activity of bovine FGF acidic and will also neutralize the biological activity of rhFGF acidic, although 5 times the amount of IgG is required.
Polyclonal Rabbit IgG
Protein A or G purified
Bovine brain-derived FGF acidic
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize FGF acidic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 0.75 ng/mL Bovine FGF acidic.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF acidic and Neutralization by Bovine FGF acidic Antibody. Bovine FGF acidic (Catalog # 132‑FA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF acidic (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti‑Bovine FGF acidic Polyclonal Antibody (Catalog # AB‑32‑NA). The ND50 is typically 1‑3 µg/mL.
Preparation and Storage
Reconstitute at 1 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF acidic
FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.
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