Bovine FGF acidic Antibody

(1 citations)   
  • Species Reactivity
    Bovine
  • Specificity
    Detects bovine FGF acidic in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize both bovine FGF acidic and recombinant human FGF acidic. In direct ELISAs, no cross‑reactivity with __________________ is observed. Neutralizes the biological activity of bovine FGF acidic and will also neutralize the biological activity of rhFGF acidic, although 5 times the amount of IgG is required.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Protein A or G purified
  • Immunogen
    Bovine brain-derived FGF acidic
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Bovine FGF acidic (Catalog # 132-FA)
  • Neutralization
    Measured by its ability to neutralize FGF acidic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 0.75 ng/mL Bovine FGF acidic.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by FGF acidic and Neutral­ization by Bovine FGF acidic Antibody. Bovine FGF acidic (Catalog # 132‑FA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF acidic (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti‑Bovine FGF acidic Polyclonal Antibody (Catalog # AB‑32‑NA). The ND50 is typically 1‑3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF acidic
FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.
  • References:
    1. Gimenez-Gallego, G. et al. (1985) Science 230:1385.
    2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
    3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
    4. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
    5. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
    6. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
    7. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
    8. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
    9. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
    10. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
    11. Imamura, T. et al. (1990) Science 249:1567.
    12. Wesche, J. et al. (2005) Biochemistry 44:6071.
    13. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
    14. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
    15. Bouleau, S. et al. (2005) Oncogene 24:7839.
  • Long Name:
    Fibroblast Growth Factor acidic
  • Entrez Gene IDs:
    2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
  • Alternate Names:
    AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF1; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Human FGF-1 gene transfer promotes the formation of collateral vessels and arterioles in ischemic muscles of hypercholesterolemic hamsters.
    Authors: Caron A, Michelet S, Caron A, Sordello S, Ivanov MA, Delaere P, Branellec D, Schwartz B, Emmanuel F
    J Gene Med, 2004;6(9):1033-45.
    Species: Hamster
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Isotype Controls
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG Antibody

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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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