Bovine FGF acidic Antibody

  (1 citations)     
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  • Species Reactivity
    Bovine
  • Specificity
    Detects bovine FGF acidic in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize both bovine FGF acidic and recombinant human (rh) FGF acidic. In direct ELISAs, no cross‑reactivity with rhFGF-basic, rhFGF-4, -5, -6, -7 or -9 is observed. Neutralizes the biological activity of bovine FGF acidic and will also neutralize the biological activity of rhFGF acidic, although 5 times the amount of IgG is required.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Protein A or G purified
  • Immunogen
    Bovine brain-derived FGF acidic
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Bovine FGF acidic (Catalog # 132-FA)
  • Neutralization
    Measured by its ability to neutralize FGF acidic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 0.75 ng/mL Bovine FGF acidic.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Cell Proliferation Induced by FGF acidic and Neutral­ization by Bovine FGF acidic Antibody. Bovine FGF acidic (Catalog # 132‑FA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF acidic (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti‑Bovine FGF acidic Polyclonal Antibody (Catalog # AB‑32‑NA). The ND50 is typically 1‑3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF acidic

FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1‑3). Bovine FGF acidic shares 53% amino acid sequence identity with FGF basic and 11%‑31% with other bovine FGFs. It shares 92%, 91%, 88%, and 91% aa sequence identity with human, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (4). It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate (5). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryognesis thereby regulate FGF acidic bioactivity (6). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (7, 8). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (9). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (10‑12). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (13, 14). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (15).

  • References:
    1. Gimenez-Gallego, G. et al. (1985) Science 230:1385.
    2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
    3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
    4. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
    5. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
    6. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
    7. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
    8. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
    9. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
    10. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
    11. Imamura, T. et al. (1990) Science 249:1567.
    12. Wesche, J. et al. (2005) Biochemistry 44:6071.
    13. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
    14. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
    15. Bouleau, S. et al. (2005) Oncogene 24:7839.
  • Long Name:
    Fibroblast Growth Factor acidic
  • Entrez Gene IDs:
    2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
  • Alternate Names:
    AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF1; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Human FGF-1 gene transfer promotes the formation of collateral vessels and arterioles in ischemic muscles of hypercholesterolemic hamsters.
    Authors: Caron A, Michelet S, Caron A, Sordello S, Ivanov MA, Delaere P, Branellec D, Schwartz B, Emmanuel F
    J Gene Med, 2004;6(9):1033-45.
    Species: Hamster
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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