c-jun [p Ser63] Antibody (4A11) - BSA Free

Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] -

Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Image of c-jun [p Ser63] Antibody (4A11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] -

Western Blot: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate (treated with Calyculin A or EGF).
All lanes: c-jun [p Ser63] Antibody at 0.95 ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 48 KDa
Observed band size: 48 KDa

Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] -

Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Staining of A549 cells with c-jun [p Ser63] Antibody (4A11) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).