Key Product Details

Species Reactivity

Canine, Feline

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 371907
View all SCF/c-kit Ligand Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine SCF/c‑kit Ligand
Lys26-Ala190
Accession # Q06220

Specificity

Detects canine and feline SCF/c‑kit Ligand in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50%‑100% cross‑reactivity with recombinant human (rh) SCF and recombinant mouse SCF is observed and no cross-reactivity with rhM-CSF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Canine/Feline SCF/c‑kit Ligand Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Canine SCF/c-kit Ligand (Catalog # 2278-SC)
Recombinant Feline SCF/c-kit Ligand (Catalog # 2268-SC)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCF/c-kit Ligand

SCF, also known as c-kit ligand, is a 28 kDa‑40 kDa type I transmembrane (TM) glycoprotein that plays important roles in fetal and adult developmental processes. Endothelial cells, fibroblasts, and keratinocytes can express SCF. The 190 aa extracellular region contains a proteolytic cleavage site, generating 165 aa circulating monomers and noncovalent homodimers. Soluble feline and canine SCF share 93% aa identity with each other and 88%‑92% aa identity with pig, cow, sheep, goat, horse, and mink SCF.

Long Name

Stem Cell Factor

Alternate Names

c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

Gene Symbol

KITLG

UniProt

Q06220

Additional SCF/c-kit Ligand Products

Product Documents for Canine/Feline SCF/c‑kit Ligand Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Canine/Feline SCF/c‑kit Ligand Antibody

For research use only

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