Canine IFN-gamma Antibody (142508) [Biotin]

Name: Canine IFN-gamma Antibody (142508) [Biotin]
Brand: R&D Systems
SKU: BAM7811
Price: 569 USD
Availability: InStock

R&D Systems | Catalog # BAM7811

Submit a review

Citations (2)

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Canine

Cited:

Canine

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development (Detection)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 142508

View all IFN-gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine IFN-gamma
Gln24-Lys166
Accession # P42161

Specificity

Detects canine IFN-gamma in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human, mouse, rat, cotton rat, feline, porcine, equine, or bovine IFN-gamma was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Canine IFN-gamma Antibody (142508) [Biotin]

Canine IFN-gamma ELISA Standard Curve

Recombinant Canine IFN‑ gamma (Catalog # 781-CG) was serially diluted and captured by Mouse Anti-Canine IFN‑ gamma Monoclonal Antibody (Catalog # MAB781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Canine IFN‑ gamma Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Canine IFN-gamma Antibody (142508) [Biotin]

Canine IFN-gamma Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:

Capture Reagent: Canine IFN‑ gamma Antibody (Catalog # MAB781)
Standard: Recombinant Canine IFN-gamma Protein (Catalog # 781-CG)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 79% - 88% amino acid sequence identity with bovine, equine, and feline IFN-gamma, 62% - 73% with human, porcine, and rhesus IFN-gamma, and 40% - 47% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).