Canine IFN-gamma Antibody

Catalog # Availability Size / Price Qty
MAB781-SP
MAB781-100
MAB781-500
Product Details
Citations (7)
FAQs
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Canine IFN-gamma Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 75% cross‑reactivity with recombinant porcine IFN-gamma and no cross-reactivity with IFN-gamma from human, mouse, feline, rat, or rhesus macaque.
Source
Monoclonal Mouse IgG2B Clone # 142529
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant canine IFN-gamma
Gln24-Lys166
Accession # P42161
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Canine IFN-gamma (Catalog # 781-CG)

Canine IFN-gamma Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Canine IFN‑ gamma Biotinylated Antibody (Catalog # BAM7811)

Standard: Recombinant Canine IFN-gamma Protein (Catalog # 781-CG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 79% - 88% amino acid sequence identity with bovine, equine, and feline IFN-gamma, 62% - 73% with human, porcine, and rhesus IFN-gamma, and 40% - 47% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Zucker, K. et al. (1992) J. Interferon Res. 12:191.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Product Datasheets

Citations for Canine IFN-gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant
    Vaccine, 2016;0(0):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs
    PLoS Negl Trop Dis, 2016;10(5):e0004614.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  3. Programmed death 1-mediated T cell exhaustion during visceral leishmaniasis impairs phagocyte function.
    Authors: Esch K, Juelsgaard R, Martinez P, Jones D, Petersen C
    J Immunol, 2013;191(11):5542-50.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial.
    Authors: Lemesre JL, Holzmuller P, Goncalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G
    Vaccine, 2007;25(21):4223-34.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  5. Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes.
    Authors: Lemesre JL, Holzmuller P, Cavaleyra M, Goncalves RB, Hottin G, Papierok G
    Vaccine, 2005;23(22):2825-40.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in Leishmania infantum-infected dogs.
    Authors: Strauss-Ayali D, Baneth G, Shor S, Okano F, Jaffe CL
    Int. J. Parasitol., 2005;35(1):63-73.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs.
    Authors: Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM
    Vaccine, 2005;24(4):501-9.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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