|VEGF in Canine PBMCs. VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine VEGF 164 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1603) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternately spliced isoforms of VEGF ranging from 206 amino acids (aa) to 121 aa in length are known. Three isoforms, VEGF188, VEGF182, and VEGF164, have been identified in canine. Canine VEGF164 shares 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline homologs, respectively. Two type I transmembrane receptor tyrosine kinases, VEGF R1 and VEGF R2, that bind VEGF with high affinity, have been identified. Neuropilin-1, a receptor for semaphorin, also binds VEGF and acts as a co‑receptor to enhance the affinity between VEGF and VEGF R2. Neuropilin-1 alone can also mediate VEGF-induced endothelial cell migration. VEGF regulates cell proliferation, migration, and survival of endothelial cells. These functions are partially mediated through the induction of nitric oxide, prostacyclin, and metalloproteinases. Together with angiopoietins or other vascular-specific growth factors, VEGF plays a separate but complementary role in angiogenesis and vasculogenesis (1‑7).
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