Canine VEGF164 Antibody Summary
Accession # Q9MYV3
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by VEGF and Neutralization by Canine VEGF Antibody. Recombinant Canine VEGF (Catalog # 1603-CV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine VEGF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine VEGF164Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603). The ND50 is typically 0.04-0.10 µg/mL.
VEGF in Canine PBMCs. VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Canine VEGF164 is an approximately 45 kDa molecular weight homodimer Canine sharing 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.
Citations for Canine VEGF164 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A165 No Longer Depends on the Growth Factor's Presence
Authors: HL Deissler, M Rehak, A Wolf
Sample Types: Cell Culture Supernates
Applications: Western Blot
Evaluation of a xenogeneic VEGF vaccine in dogs with soft tissue sarcoma.
Authors: Kamstock D, Elmslie R, Thamm D
Cancer Immunol. Immunother., 2007;56(8):1299-309.
Sample Types: Whole Tissue
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