Key Product Details

Species Reactivity

Validated:

Canine

Cited:

Canine

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Immunocytochemistry

Cited:

Immunohistochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 247109
View all VEGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine VEGF164
Pro28-Arg190
Accession # NP_001103972

Specificity

Detects canine VEGF in ELISAs and Western blots. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) VEGF

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Canine VEGF Antibody

VEGF antibody in Canine PBMCs by Immunocytochemistry (ICC).

VEGF in Canine PBMCs.

VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Canine VEGF Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells (PBMCs)

Western Blot

1 µg/mL
Sample: Recombinant Canine VEGF (Catalog # 1603-CV)

Canine VEGF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Canine VEGF164 Biotinylated Antibody (Catalog # BAF1603)
  • Standard: Recombinant Canine VEGF Protein (Catalog # 1603-CV)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Canine VEGF164 is an approximately 45 kDa molecular weight homodimer Canine sharing 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

UniProt

NP_001103972

Additional VEGF Products

Product Documents for Canine VEGF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Canine VEGF Antibody

For research use only

Citations for Canine VEGF Antibody

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Protocols

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