Detects canine VEGF in ELISAs and Western blots. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) VEGF121 and rhVEGF165, less than 25% cross-reactivity with recombinant rat VEGF164, and no cross-reactivity with rhVEGF-B186, rhVEGF-C, rhVEGF‑D, recombinant mouse (rm) VEGF115, rmVEGF120, rmVEGF165, or recombinant zebrafish VEGF165 is observed.
Monoclonal Mouse IgG1 Clone # 247109
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant canine VEGF164 Pro28-Arg190 Accession # NP_001103972
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
VEGF in Canine PBMCs.
VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternately spliced isoforms of VEGF ranging from 206 amino acids (aa) to 121 aa in length are known. Three isoforms, VEGF188, VEGF182, and VEGF164, have been identified in canine. Canine VEGF164 shares 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline homologs, respectively. Two type I transmembrane receptor tyrosine kinases, VEGF R1 and VEGF R2, that bind VEGF with high affinity, have been identified. Neuropilin-1, a receptor for semaphorin, also binds VEGF and acts as a co-receptor to enhance the affinity between VEGF and VEGF R2. Neuropilin-1 alone can also mediate VEGF-induced endothelial cell migration. VEGF regulates cell proliferation, migration, and survival of endothelial cells. These functions are partially mediated through the induction of nitric oxide, prostacyclin, and metalloproteinases. Together with angiopoietins or other vascular-specific growth factors, VEGF plays a separate but complementary role in angiogenesis and vasculogenesis (1-7).
JingJing, L. et al. (2000) Mol. Cell Biochem. 214:23.
Scheidegger, P. et al. (1999) Biol. Chem. 380:1449.
Thurston, G. (2002) J. Anat. 200:575.
Zachary, I. and G. Gliki (2001) Cardiovasc. Res. 49:568.
Chevalier, S. (2002) Mol. Cell Endocrinol. 189:169.
Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.