CD117/c-kit Antibody (2B8) - BSA Free

Novus Biologicals | Catalog # NB100-77477

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # 2B8

Format

BSA Free
View all CD117/c-kit Primary Antibodies »

Product Specifications

Immunogen

Mouse bone marrow mast cells

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23967247).

Marker

Hematopoietic Stem Cell Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for CD117/c-kit Antibody (2B8) - BSA Free

Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]

Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]

Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).
Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]

Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]

CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0004.jpg
Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]

Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477]

Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC.
CD117/c-kit Antibody (2B8) - BSA Free

Flow Cytometry: CD117/c-kit Antibody (2B8) - BSA Free [NB100-77477] -

Identification of c-kit expressing bone marrow cells.Double labeling of mouse bone marrow cells with anti-CD45 antibody and (a) Kit-129 aptamer or (b) rabbit anti-c-kit antibody 2B8.

Applications for CD117/c-kit Antibody (2B8) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.6 ug/10^6 cells in 100 uL

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 28097227)

Immunohistochemistry

1:20 - 1:1000

Immunohistochemistry-Frozen

1:20 - 1:1000

Immunoprecipitation

1:20 - 1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

Reviewed Applications

Read 1 review rated 1 using NB100-77477 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (2B8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD117/c-kit Antibody (2B8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD117/c-kit Antibody (2B8) - BSA Free

Customer Reviews for CD117/c-kit Antibody (2B8) - BSA Free (1)

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  • CD117/c-kit Antibody (2B8)
    Name: Fumihito Noguchi
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human skin
    Species: Human
    Verified Customer | Posted 01/02/2018
    We tested CD117/c-kit antibody (2B8) for IHC-P (human skin) and confirmed that it did not work to detect human melanocytes which are reportedly positive for ckit, indicating that this product is not applicable for FFPE section.
    CD117/c-kit Antibody (2B8) - BSA Free NB100-77477

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