CD117/c-kit Antibody (2B8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80624
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # 2B8
Format
Azide and BSA Free
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Product Specifications
Immunogen
Mouse bone marrow mast cells
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 23967247).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Scientific Data Images for CD117/c-kit Antibody (2B8) - Azide and BSA Free
![Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624]](https://resources.rndsystems.com/images/products/CD117-c-kit-Antibody-2B8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80624-img0002.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624]")
Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624]
Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113). Image from the standard format of this antibody.![Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624]](https://resources.rndsystems.com/images/products/CD117-c-kit-Antibody-2B8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80624-img0001.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624]")
Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624]
Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC. Image from the standard format of this antibody.![Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624]](https://resources.rndsystems.com/images/products/CD117-c-kit-Antibody-2B8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80624-img0003.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624]")
Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624]
Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - Identification of CD117/c-kit expressing bone marrow cells.Double labeling of mouse bone marrow cells with anti-CD45 antibody and rabbit anti-CD117/c-kit antibody 2B8. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0071798) licensed under a CC-BY license. Image from the standard format of this antibody.Applications for CD117/c-kit Antibody (2B8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.6 ug/10^6 cells in 100 uL
Immunocytochemistry/ Immunofluorescence
0.25 ug/10^6 cells in 100 uL. Use reported in scientific literature (PMID 28097227)
Immunohistochemistry
1:20 - 1:1000
Immunohistochemistry-Frozen
1:20 - 1:1000
Immunoprecipitation
1:20 - 1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD117/c-kit
Long Name
Stem Cell Factor Receptor
Alternate Names
c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR
Gene Symbol
KIT
Additional CD117/c-kit Products
Product Documents for CD117/c-kit Antibody (2B8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD117/c-kit Antibody (2B8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
