CD117/c-kit Antibody (ACK2) - BSA Free

Novus Biologicals | Catalog # NBP1-43359

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Guinea Pig

Cited:

Human, Mouse, Rat, Guinea Pig

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # ACK2

Format

BSA Free
View all CD117/c-kit Primary Antibodies »

Product Specifications

Immunogen

Mouse CD117

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23967247). Guinea Pig reactivity reported in scientific literature (PMID: 21654812)

Marker

Hematopoietic Stem Cell Marker

Specificity

The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Description

Novus Biologicals Rat CD117/c-kit Antibody (ACK2) - BSA Free (NBP1-43359) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-CD117/c-kit Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD117/c-kit Antibody (ACK2) - BSA Free

Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]

Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]

CD117-c-kit-Antibody-ACK2-Immunohistochemistry-Whole-Mount-NBP1-43359-img0003.jpg
Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]

Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]

Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of Allophycocyanin conjugate of NBP1-43359. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b kappa Isotype Control APC (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) APC (right). Total viable c
Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]

Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]

Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype Control PE-Cy5 (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) PE-Cy5 (right). Total viable cells were

Applications for CD117/c-kit Antibody (ACK2) - BSA Free

Application
Recommended Usage

Flow Cytometry

< 0.125 ug/10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID 26680736). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24161026).

Reviewed Applications

Read 1 review rated 3 using NBP1-43359 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD117/c-kit

The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human)

Gene Symbol

KIT

UniProt

P10721

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (ACK2) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD117/c-kit Antibody (ACK2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD117/c-kit Antibody (ACK2) - BSA Free

Customer Reviews for CD117/c-kit Antibody (ACK2) - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mouse Intestine (Ileum)
    Species: Mouse
    Verified Customer | Posted 09/02/2015
    ACK2/CD117 in a live intestinal slice 48h ex vivo. Antibody was added in vitro, prior to fixing and running with secondary.
    CD117/c-kit Antibody (ACK2) - BSA Free NBP1-43359

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Protocols

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FAQs for CD117/c-kit Antibody (ACK2) - BSA Free

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    • Q: Is there a blocking peptide that can be used as a control with the c-Kit antibody (ACK2) (NBP1-43359)?
      A: NBP1-46164, NBP1-46249, P4713, P4834, H00003815-P01 are all c-kit proteins that could be suitable for blocking purposes.
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