CD117/c-kit Antibody (OTI2B12)

Name: CD117/c-kit Antibody (OTI2B12)
Brand: Novus Biologicals
SKU: NBP2-45508
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-45508

Submit a review

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # OTI2B12

View all CD117/c-kit Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 27-308 of human KIT(NP_000213) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

109.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD117/c-kit Antibody (OTI2B12)

Western Blot: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Western Blot: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD117.

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) [NBP2-45508]

Immunohistochemistry: CD117/c-kit Antibody (2B12) [NBP2-45508] - Analysis of Carcinoma of Human bladder tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

View 10 more images

Applications for CD117/c-kit Antibody (OTI2B12)

Application

Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (OTI2B12)

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD117/c-kit Antibody (OTI2B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.