Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2B12

Format

Azide and BSA Free
View all CD117/c-kit Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 27-308 of human KIT(NP_000213) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

109.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free (NBP2-45508) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free

Western Blot: CD117/c-kit Antibody (OTI2B12)Azide and BSA Free [NBP2-71076]

Western Blot: CD117/c-kit Antibody (OTI2B12)Azide and BSA Free [NBP2-71076]

Western Blot: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIT ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIT (1:2000). Positive lysates.
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Human colon tissue within the normal limits using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Adenocarcinoma of Human colon tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Carcinoma of Human thyroid tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Human lymphoma tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3min.
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human bladder tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076]

Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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