CD133 Antibody [DyLight 488]

Novus Biologicals | Catalog # NB120-16518G

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Cited:

Flow Cytometry

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Rabbit IgG
View all CD133 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).

Reactivity Notes

porcine reactivity reported in scientific literature (PMID: 29352176).

Marker

Stem Cell Marker

Specificity

CD133 - Hematopoietic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Scientific Data Images for CD133 Antibody [DyLight 488]

CD133 Antibody [DyLight 488]-Immunocytochemistry-Immunofluorescence-NB120-16518G-img0003.jpg
CD133-Antibody-[DyLight-488]-Flow-Cytometry-NB120-16518G-img0002.jpg
Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed.
CD133 Antibody [DyLight 488]

Immunocytochemistry/Immunofluorescence: CD133 Antibody [DyLight 488] [NB120-16518G] -

Characterization of Scattered tubular-like cells (STCs) isolated from pig kidneys. (A) Representative immunofluorescence staining (original magnification 40X) for the STC surface markers CD133 (red) and CD24 (green) in isolated swine STCs. (B) Flow cytometry analysis of isolated STCs showing that 97% of cells expressed CD133, 95% CD24, and 98% were double-positive for CD133 and CD24 (n = 3 each).
CD133 Antibody [DyLight 488]

Immunocytochemistry/ Immunofluorescence: CD133 Antibody [DyLight 488] [NB120-16518G] -

Immunocytochemistry/ Immunofluorescence: CD133 Antibody [DyLight 488] [NB120-16518G] - Characterization of Scattered tubular-like cells (STCs) isolated from pig kidneys. (A) Representative immunofluorescence staining (original magnification 40X) for the STC surface markers CD133 (red) & CD24 (green) in isolated swine STCs. (B) Flow cytometry analysis of isolated STCs showing that 97% of cells expressed CD133, 95% CD24, & 98% were double-positive for CD133 & CD24 (n = 3 each). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31614781), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CD133 Antibody [DyLight 488]

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: 1/1000. WB: 1/1,000 - 1:2,000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user.

Reviewed Applications

Read 1 review rated 4 using NB120-16518G in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD133

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody [DyLight 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD133 Antibody [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD133 Antibody [DyLight 488]

Customer Reviews for CD133 Antibody [DyLight 488] (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used CD133 Antibody [DyLight 488]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • CD133 Antibody [DyLight 488]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Bone marrow cells
    Species: Rat
    Verified Customer | Posted 05/30/2018
    Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed.
    CD133 Antibody [DyLight 488] NB120-16518G

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies