CD25/IL-2R alpha Antibody (1B5D12) - BSA Free

Name: CD25/IL-2R alpha Antibody (1B5D12) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-37349
Price: 199 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-37349

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1B5D12

Format

BSA Free

View all CD25/IL-2R alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD25/IL-2R alpha (AA: 34-139) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

30.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD25/IL-2R alpha Antibody (1B5D12) - BSA Free

Western Blot: CD25/IL-2R alpha Antibody (1B5D12)BSA Free [NBP2-37349]

Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.

Immunocytochemistry/ Immunofluorescence: CD25/IL-2R alpha Antibody (1B5D12) - BSA Free [NBP2-37349]

Immunocytochemistry/Immunofluorescence: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of Hela cells using IL2RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) - BSA Free [NBP2-37349]

Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of bladder cancer tissues using IL2RA mouse mAb with DAB staining.

Western Blot: CD25/IL-2R alpha Antibody (1B5D12)BSA Free [NBP2-37349]

Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)

Western Blot: CD25/IL-2R alpha Antibody (1B5D12)BSA Free [NBP2-37349]

Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) - BSA Free [NBP2-37349]

Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of rectum cancer tissues using IL2RA mouse mAb with DAB staining.

ELISA: CD25/IL-2R alpha Antibody (1B5D12) - BSA Free [NBP2-37349]

ELISA: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

View 4 more images

Applications for CD25/IL-2R alpha Antibody (1B5D12) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for CD25/IL-2R alpha Antibody (1B5D12) - BSA Free

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Certificate of Analysis

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Product Specific Notices for CD25/IL-2R alpha Antibody (1B5D12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.